Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function

Yue He; Candice Yam; Kyle Pomraning; Jacqueline S R Chin; Joanne Y Yew; Michael Freitag; Snezhana Oliferenko

doi:10.1091/mbc.E14-03-0832

Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function

Yue He, Candice Yam, Kyle Pomraning, Jacqueline S R Chin, Joanne Y Yew, Michael Freitag, Snezhana Oliferenko

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in specialized cellular organelles, called lipid droplets. Understanding what determines the cellular amount of neutral lipids and their packaging into lipid droplets is of fundamental and applied interest. Using two species of fission yeast, we show that cycling cells deficient in the function of the ER-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. We demonstrate that these unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Our results suggest that interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway.

Original language	English
Pages (from-to)	4083-4095
Number of pages	13
Journal	Molecular biology of the cell
Volume	25
Issue number	25
DOIs	https://doi.org/10.1091/mbc.E14-03-0832
Publication status	Published - 15 Oct 2014

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title = "Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function",

abstract = "Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in specialized cellular organelles, called lipid droplets. Understanding what determines the cellular amount of neutral lipids and their packaging into lipid droplets is of fundamental and applied interest. Using two species of fission yeast, we show that cycling cells deficient in the function of the ER-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. We demonstrate that these unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Our results suggest that interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway.",

author = "Yue He and Candice Yam and Kyle Pomraning and Chin, {Jacqueline S R} and Yew, {Joanne Y} and Michael Freitag and Snezhana Oliferenko",

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T1 - Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function

AU - He, Yue

AU - Yam, Candice

AU - Pomraning, Kyle

AU - Chin, Jacqueline S R

AU - Yew, Joanne Y

AU - Freitag, Michael

AU - Oliferenko, Snezhana

PY - 2014/10/15

Y1 - 2014/10/15

N2 - Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in specialized cellular organelles, called lipid droplets. Understanding what determines the cellular amount of neutral lipids and their packaging into lipid droplets is of fundamental and applied interest. Using two species of fission yeast, we show that cycling cells deficient in the function of the ER-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. We demonstrate that these unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Our results suggest that interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway.

AB - Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in specialized cellular organelles, called lipid droplets. Understanding what determines the cellular amount of neutral lipids and their packaging into lipid droplets is of fundamental and applied interest. Using two species of fission yeast, we show that cycling cells deficient in the function of the ER-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. We demonstrate that these unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Our results suggest that interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway.

U2 - 10.1091/mbc.E14-03-0832

DO - 10.1091/mbc.E14-03-0832

M3 - Article

C2 - 25318672

SN - 1939-4586

VL - 25

SP - 4083

EP - 4095

JO - Molecular biology of the cell

JF - Molecular biology of the cell

IS - 25

ER -

Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function

Abstract

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