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Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients

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Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients. / www.GAMBITstudy.co.uk ; Lord, Graham Michael; Lechler, Robert Ian; Lombardi, Giovanna.

In: Transplantation, 28.07.2016.

Research output: Contribution to journalArticle

Harvard

www.GAMBITstudy.co.uk, Lord, GM, Lechler, RI & Lombardi, G 2016, 'Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients', Transplantation. https://doi.org/10.1097/TP.0000000000001341

APA

www.GAMBITstudy.co.uk, Lord, G. M., Lechler, R. I., & Lombardi, G. (2016). Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients. Transplantation, [TPA-2016-0165R1 ]. https://doi.org/10.1097/TP.0000000000001341

Vancouver

www.GAMBITstudy.co.uk, Lord GM, Lechler RI, Lombardi G. Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients. Transplantation. 2016 Jul 28. TPA-2016-0165R1 . https://doi.org/10.1097/TP.0000000000001341

Author

www.GAMBITstudy.co.uk ; Lord, Graham Michael ; Lechler, Robert Ian ; Lombardi, Giovanna. / Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients. In: Transplantation. 2016.

Bibtex Download

@article{a24e02601e1d47cd8dc82b8815ac10b1,
title = "Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients",
abstract = "BACKGROUND: An increased percentage of peripheral transitional B cells producing IL-10 has been observed in patients tolerant to kidney allografts. In healthy volunteers, the balance between the CD40 and B cell receptor (BCR) signalling modulated IL-10 production by B cells, with stimulation via the BCR decreasing CD40-mediated-IL-10 production. In this study, we evaluate whether in tolerant kidney transplant patients the increased IL-10 production by B cells was due to an altered CD40 and/or BCR signalling.METHODS: B cells obtained from a new cohort of tolerant renal transplant recipients and those from age- and gender-matched healthy volunteers, were activated via CD40 and BCR, either alone or in combination.RESULTS: In tolerant patients we observed higher percentages of B cells producing IL-10 after CD40 ligation and higher expression of CD40L on activated T cells, compared to healthy controls. Furthermore, B cells from tolerant recipients had reduced ERK signalling following BCR-mediated activation compared to healthy controls. In keeping with this, combining BCR signalling with CD40 ligation did not reduce IL-10 secretion as was observed in healthy control transitional B cells.CONCLUSION: Altogether our data suggests that the altered response of B cells in tolerant recipients may contribute to long-term stable graft acceptance.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially.",
author = "{Nova Lamperti}, {Estefania Andrea} and Prabhjoat Chana and Mobillo, {Paula Andrea} and Manohursingh Runglall and Kamra, {Yogesh Kishore} and McGregor, {Reuben Hendrick Cameron} and www.GAMBITstudy.co.uk and Lord, {Graham Michael} and Lechler, {Robert Ian} and Giovanna Lombardi and {Hernandez Fuentes}, {Maria Puerto}",
year = "2016",
month = "7",
day = "28",
doi = "10.1097/TP.0000000000001341",
language = "English",
journal = "Transplantation",
issn = "0041-1337",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Increased CD40 ligation and reduced BCR signalling leads to higher IL-10 production in B-cells from tolerant kidney transplant patients

AU - Nova Lamperti, Estefania Andrea

AU - Chana, Prabhjoat

AU - Mobillo, Paula Andrea

AU - Runglall, Manohursingh

AU - Kamra, Yogesh Kishore

AU - McGregor, Reuben Hendrick Cameron

AU - www.GAMBITstudy.co.uk

AU - Lord, Graham Michael

AU - Lechler, Robert Ian

AU - Lombardi, Giovanna

AU - Hernandez Fuentes, Maria Puerto

PY - 2016/7/28

Y1 - 2016/7/28

N2 - BACKGROUND: An increased percentage of peripheral transitional B cells producing IL-10 has been observed in patients tolerant to kidney allografts. In healthy volunteers, the balance between the CD40 and B cell receptor (BCR) signalling modulated IL-10 production by B cells, with stimulation via the BCR decreasing CD40-mediated-IL-10 production. In this study, we evaluate whether in tolerant kidney transplant patients the increased IL-10 production by B cells was due to an altered CD40 and/or BCR signalling.METHODS: B cells obtained from a new cohort of tolerant renal transplant recipients and those from age- and gender-matched healthy volunteers, were activated via CD40 and BCR, either alone or in combination.RESULTS: In tolerant patients we observed higher percentages of B cells producing IL-10 after CD40 ligation and higher expression of CD40L on activated T cells, compared to healthy controls. Furthermore, B cells from tolerant recipients had reduced ERK signalling following BCR-mediated activation compared to healthy controls. In keeping with this, combining BCR signalling with CD40 ligation did not reduce IL-10 secretion as was observed in healthy control transitional B cells.CONCLUSION: Altogether our data suggests that the altered response of B cells in tolerant recipients may contribute to long-term stable graft acceptance.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially.

AB - BACKGROUND: An increased percentage of peripheral transitional B cells producing IL-10 has been observed in patients tolerant to kidney allografts. In healthy volunteers, the balance between the CD40 and B cell receptor (BCR) signalling modulated IL-10 production by B cells, with stimulation via the BCR decreasing CD40-mediated-IL-10 production. In this study, we evaluate whether in tolerant kidney transplant patients the increased IL-10 production by B cells was due to an altered CD40 and/or BCR signalling.METHODS: B cells obtained from a new cohort of tolerant renal transplant recipients and those from age- and gender-matched healthy volunteers, were activated via CD40 and BCR, either alone or in combination.RESULTS: In tolerant patients we observed higher percentages of B cells producing IL-10 after CD40 ligation and higher expression of CD40L on activated T cells, compared to healthy controls. Furthermore, B cells from tolerant recipients had reduced ERK signalling following BCR-mediated activation compared to healthy controls. In keeping with this, combining BCR signalling with CD40 ligation did not reduce IL-10 secretion as was observed in healthy control transitional B cells.CONCLUSION: Altogether our data suggests that the altered response of B cells in tolerant recipients may contribute to long-term stable graft acceptance.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially.

U2 - 10.1097/TP.0000000000001341

DO - 10.1097/TP.0000000000001341

M3 - Article

C2 - 27472092

JO - Transplantation

JF - Transplantation

SN - 0041-1337

M1 - TPA-2016-0165R1

ER -

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