Individual Limb Muscle Bundles Are Formed through Progressive Steps Orchestrated by Adjacent Connective Tissue Cells during Primary Myogenesis

TY - JOUR

T1 - Individual Limb Muscle Bundles Are Formed through Progressive Steps Orchestrated by Adjacent Connective Tissue Cells during Primary Myogenesis

AU - Besse, Laurianne

AU - Sheeba, Caroline J.

AU - Holt, Mark

AU - Labuhn, Maurice

AU - Wilde, Susan

AU - Feneck, Eleanor

AU - Bell, Donald

AU - Kucharska, Ania

AU - Logan, Malcolm P.O.

PY - 2020/3/10

Y1 - 2020/3/10

N2 - Although the factors regulating muscle cell differentiation are well described, we know very little about how differentiating muscle fibers are organized into individual muscle tissue bundles. Disruption of these processes leads to muscle hypoplasia or dysplasia, and replicating these events is vital in tissue engineering approaches. We describe the progressive cellular events that orchestrate the formation of individual limb muscle bundles and directly demonstrate the role of the connective tissue cells that surround muscle precursors in controlling these events. We show how disruption of gene activity within or genetic ablation of connective tissue cells impacts muscle precursors causing disruption of muscle bundle formation and subsequent muscle dysplasia and hypoplasia. We identify several markers of the populations of connective tissue cells that surround muscle precursors and provide a model for how matrix-modifying proteoglycans secreted by these cells may influence muscle bundle formation by effects on the local extracellular matrix (ECM) environment.

AB - Although the factors regulating muscle cell differentiation are well described, we know very little about how differentiating muscle fibers are organized into individual muscle tissue bundles. Disruption of these processes leads to muscle hypoplasia or dysplasia, and replicating these events is vital in tissue engineering approaches. We describe the progressive cellular events that orchestrate the formation of individual limb muscle bundles and directly demonstrate the role of the connective tissue cells that surround muscle precursors in controlling these events. We show how disruption of gene activity within or genetic ablation of connective tissue cells impacts muscle precursors causing disruption of muscle bundle formation and subsequent muscle dysplasia and hypoplasia. We identify several markers of the populations of connective tissue cells that surround muscle precursors and provide a model for how matrix-modifying proteoglycans secreted by these cells may influence muscle bundle formation by effects on the local extracellular matrix (ECM) environment.

KW - Holt-Oram syndrome

KW - HOS

KW - irregular connective tissue

KW - muscle

KW - muscle connective tissue

KW - Tbx5

KW - tissue morphogenesis

UR - http://www.scopus.com/inward/record.url?scp=85081015559&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2020.02.037

DO - 10.1016/j.celrep.2020.02.037

M3 - Article

AN - SCOPUS:85081015559

SN - 2211-1247

VL - 30

SP - 3552-3565.e6

JO - Cell Reports

JF - Cell Reports

IS - 10

ER -