Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)

Sandra Ropret; Jakob Jeriha; Tjaša Sorčan; Marija Rogar; Špela Zemljič Jokhadar; John A. McGrath; Dusko Ilic; Mirjana Liovic

doi:10.1016/j.scr.2021.102463

Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)

Sandra Ropret, Jakob Jeriha, Tjaša Sorčan, Marija Rogar, Špela Zemljič Jokhadar, John A. McGrath, Dusko Ilic, Mirjana Liovic^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts.

Original language	English
Article number	102463
Journal	Stem Cell Research
Volume	55
DOIs	https://doi.org/10.1016/j.scr.2021.102463
Publication status	Published - Aug 2021

Keywords

Collagen VII
Fibroblast
iPSC line
Keratinocyte
Pluripotency
Recessive dystrophic EB

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10.1016/j.scr.2021.102463

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@article{f1561d7cae5043799d415d7f594d4673,

title = "Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)",

abstract = "We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts.",

keywords = "Collagen VII, Fibroblast, iPSC line, Keratinocyte, Pluripotency, Recessive dystrophic EB",

author = "Sandra Ropret and Jakob Jeriha and Tja{\v s}a Sor{\v c}an and Marija Rogar and {Zemlji{\v c} Jokhadar}, {\v S}pela and McGrath, {John A.} and Dusko Ilic and Mirjana Liovic",

note = "Funding Information: This work was supported by an EMBO Short-Term Fellowship to M. Liovic, the EraCoSysMed 4D-HEALING grant, and the Celsa Alliance grant to M. Liovic. Publisher Copyright: {\textcopyright} 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = aug,

doi = "10.1016/j.scr.2021.102463",

language = "English",

volume = "55",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)

AU - Ropret, Sandra

AU - Jeriha, Jakob

AU - Sorčan, Tjaša

AU - Rogar, Marija

AU - Zemljič Jokhadar, Špela

AU - McGrath, John A.

AU - Ilic, Dusko

AU - Liovic, Mirjana

N1 - Funding Information: This work was supported by an EMBO Short-Term Fellowship to M. Liovic, the EraCoSysMed 4D-HEALING grant, and the Celsa Alliance grant to M. Liovic. Publisher Copyright: © 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/8

Y1 - 2021/8

N2 - We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts.

AB - We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts.

KW - Collagen VII

KW - Fibroblast

KW - iPSC line

KW - Keratinocyte

KW - Pluripotency

KW - Recessive dystrophic EB

UR - http://www.scopus.com/inward/record.url?scp=85110391206&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2021.102463

DO - 10.1016/j.scr.2021.102463

M3 - Article

AN - SCOPUS:85110391206

SN - 1873-5061

VL - 55

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102463

ER -

Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)

Abstract

Keywords

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