Influence of folate status on genomic DNA methylation in colonic mucosa of subjects without colorectal adenoma or cancer

M Pufulete; R Al-Ghnaniem; J  A Rennie; P Appleby; N Harris; S Gout; P  W Emery; T  A Sanders

doi:10.1038/sj.bjc.6602439

Influence of folate status on genomic DNA methylation in colonic mucosa of subjects without colorectal adenoma or cancer

M Pufulete, R Al-Ghnaniem, J A Rennie, P Appleby, N Harris, S Gout, P W Emery, T A Sanders

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Abstract

DNA hypomethylation may increase the risk of colorectal cancer. The main aim of this study was to assess the influence of folate status (serum and erythrocyte folate and plasma homocysteine concentrations) on DNA methylation. Methylenetetrahydrofolate reductase (MTHFR 677C-->T and 1298A-->C), methionine synthase (MS 2756A-->G) and cystathionine synthase (CBS 844ins68) polymorphisms were measured to account for potential confounding effects on folate status and DNA methylation. A total of 68 subjects (33 men and 35 women, 36-78 years) free from colorectal polyps or cancer were recruited in a cross-sectional study. Tissue biopsies were obtained at colonoscopy for the determination of DNA methylation in colonic mucosa using an in vitro radiolabelled methyl acceptance assay. Serum and erythrocyte folate were inversely correlated with plasma homocysteine (r = -0.573, P

Original language	English
Pages (from-to)	838 - 842
Number of pages	5
Journal	BJC: British Journal of Cancer
Volume	92
Issue number	5
DOIs	https://doi.org/10.1038/sj.bjc.6602439
Publication status	Published - 14 Mar 2005

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title = "Influence of folate status on genomic DNA methylation in colonic mucosa of subjects without colorectal adenoma or cancer",

abstract = "DNA hypomethylation may increase the risk of colorectal cancer. The main aim of this study was to assess the influence of folate status (serum and erythrocyte folate and plasma homocysteine concentrations) on DNA methylation. Methylenetetrahydrofolate reductase (MTHFR 677C-->T and 1298A-->C), methionine synthase (MS 2756A-->G) and cystathionine synthase (CBS 844ins68) polymorphisms were measured to account for potential confounding effects on folate status and DNA methylation. A total of 68 subjects (33 men and 35 women, 36-78 years) free from colorectal polyps or cancer were recruited in a cross-sectional study. Tissue biopsies were obtained at colonoscopy for the determination of DNA methylation in colonic mucosa using an in vitro radiolabelled methyl acceptance assay. Serum and erythrocyte folate were inversely correlated with plasma homocysteine (r = -0.573, P",

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T1 - Influence of folate status on genomic DNA methylation in colonic mucosa of subjects without colorectal adenoma or cancer

AU - Pufulete, M

AU - Al-Ghnaniem, R

AU - Rennie, J A

AU - Appleby, P

AU - Harris, N

AU - Gout, S

AU - Emery, P W

AU - Sanders, T A

PY - 2005/3/14

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N2 - DNA hypomethylation may increase the risk of colorectal cancer. The main aim of this study was to assess the influence of folate status (serum and erythrocyte folate and plasma homocysteine concentrations) on DNA methylation. Methylenetetrahydrofolate reductase (MTHFR 677C-->T and 1298A-->C), methionine synthase (MS 2756A-->G) and cystathionine synthase (CBS 844ins68) polymorphisms were measured to account for potential confounding effects on folate status and DNA methylation. A total of 68 subjects (33 men and 35 women, 36-78 years) free from colorectal polyps or cancer were recruited in a cross-sectional study. Tissue biopsies were obtained at colonoscopy for the determination of DNA methylation in colonic mucosa using an in vitro radiolabelled methyl acceptance assay. Serum and erythrocyte folate were inversely correlated with plasma homocysteine (r = -0.573, P

AB - DNA hypomethylation may increase the risk of colorectal cancer. The main aim of this study was to assess the influence of folate status (serum and erythrocyte folate and plasma homocysteine concentrations) on DNA methylation. Methylenetetrahydrofolate reductase (MTHFR 677C-->T and 1298A-->C), methionine synthase (MS 2756A-->G) and cystathionine synthase (CBS 844ins68) polymorphisms were measured to account for potential confounding effects on folate status and DNA methylation. A total of 68 subjects (33 men and 35 women, 36-78 years) free from colorectal polyps or cancer were recruited in a cross-sectional study. Tissue biopsies were obtained at colonoscopy for the determination of DNA methylation in colonic mucosa using an in vitro radiolabelled methyl acceptance assay. Serum and erythrocyte folate were inversely correlated with plasma homocysteine (r = -0.573, P

U2 - 10.1038/sj.bjc.6602439

DO - 10.1038/sj.bjc.6602439

M3 - Article

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JO - BJC: British Journal of Cancer

JF - BJC: British Journal of Cancer

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ER -

Influence of folate status on genomic DNA methylation in colonic mucosa of subjects without colorectal adenoma or cancer

Abstract

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