Inheritance of recombinant carp beta-actin GH cDNA gene in transgenic carp

B Moav; Y Hinits; Y Groll; S Rothbard

Inheritance of recombinant carp beta-actin GH cDNA gene in transgenic carp

B Moav^*, Y Hinits, Y Groll, S Rothbard

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Individual carp (Cyprinus carpio) raised from zygotes that were microinjected with two different carp a-actin promoters fused to the chinook salmon GH cDNA gene. Twenty five individual fish, out of 200 males and females, were shown to contain the constructs in a mosaic fashion at sexual maturity, and some of them were crossed with each other as well as with non-transgenic carp.

The level of transgene transmittance from the transgenic parents varied between individuals. We observed 32%-87% inheritance when transgenic parents were crossed and 0%-50% inheritance when transgenic parents were mated with non-transgenics.

Original language	English
Pages (from-to)	179-185
Number of pages	7
Journal	AQUACULTURE
Volume	137
Issue number	1-4
Publication status	Published - 1 Dec 1995
Event	5th International Symposium on Genetics in Aquaculture - HALIFAX, Canada Duration: 1 Jun 1994 → …

Keywords

transgenic fish
Cyprinus carpio
inheritance
growth hormone
GERM-LINE TRANSMISSION
TROUT EGGS
COMMON CARP
EXPRESSION
FISH
INTEGRATION
PROMOTERS
DNA

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title = "Inheritance of recombinant carp beta-actin GH cDNA gene in transgenic carp",

abstract = "Individual carp (Cyprinus carpio) raised from zygotes that were microinjected with two different carp a-actin promoters fused to the chinook salmon GH cDNA gene. Twenty five individual fish, out of 200 males and females, were shown to contain the constructs in a mosaic fashion at sexual maturity, and some of them were crossed with each other as well as with non-transgenic carp.The level of transgene transmittance from the transgenic parents varied between individuals. We observed 32%-87% inheritance when transgenic parents were crossed and 0%-50% inheritance when transgenic parents were mated with non-transgenics.",

keywords = "transgenic fish, Cyprinus carpio, inheritance, growth hormone, GERM-LINE TRANSMISSION, TROUT EGGS, COMMON CARP, EXPRESSION, FISH, INTEGRATION, PROMOTERS, DNA",

author = "B Moav and Y Hinits and Y Groll and S Rothbard",

year = "1995",

month = dec,

day = "1",

language = "English",

volume = "137",

pages = "179--185",

journal = "AQUACULTURE",

issn = "0044-8486",

publisher = "Elsevier",

number = "1-4",

note = "5th International Symposium on Genetics in Aquaculture ; Conference date: 01-06-1994",

}

TY - JOUR

T1 - Inheritance of recombinant carp beta-actin GH cDNA gene in transgenic carp

AU - Moav, B

AU - Hinits, Y

AU - Groll, Y

AU - Rothbard, S

PY - 1995/12/1

Y1 - 1995/12/1

N2 - Individual carp (Cyprinus carpio) raised from zygotes that were microinjected with two different carp a-actin promoters fused to the chinook salmon GH cDNA gene. Twenty five individual fish, out of 200 males and females, were shown to contain the constructs in a mosaic fashion at sexual maturity, and some of them were crossed with each other as well as with non-transgenic carp.The level of transgene transmittance from the transgenic parents varied between individuals. We observed 32%-87% inheritance when transgenic parents were crossed and 0%-50% inheritance when transgenic parents were mated with non-transgenics.

AB - Individual carp (Cyprinus carpio) raised from zygotes that were microinjected with two different carp a-actin promoters fused to the chinook salmon GH cDNA gene. Twenty five individual fish, out of 200 males and females, were shown to contain the constructs in a mosaic fashion at sexual maturity, and some of them were crossed with each other as well as with non-transgenic carp.The level of transgene transmittance from the transgenic parents varied between individuals. We observed 32%-87% inheritance when transgenic parents were crossed and 0%-50% inheritance when transgenic parents were mated with non-transgenics.

KW - transgenic fish

KW - Cyprinus carpio

KW - inheritance

KW - growth hormone

KW - GERM-LINE TRANSMISSION

KW - TROUT EGGS

KW - COMMON CARP

KW - EXPRESSION

KW - FISH

KW - INTEGRATION

KW - PROMOTERS

KW - DNA

M3 - Article

SN - 0044-8486

VL - 137

SP - 179

EP - 185

JO - AQUACULTURE

JF - AQUACULTURE

IS - 1-4

T2 - 5th International Symposium on Genetics in Aquaculture

Y2 - 1 June 1994

ER -

Inheritance of recombinant carp beta-actin GH cDNA gene in transgenic carp

Abstract

Keywords

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