Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection

Cong Wang, Chenlei Gu, Courtney Popp, Priya Vashisth, Salman Ahmad Mustfa, Davide Alessandro Martella, Chantelle Spiteri, Samuel McLennan, Ningjia Sun, Megan Riddle, Cindy R. Eide, Maddy Parsons, Jakub Tolar, John A. McGrath, Ciro Chiappini*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell-nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.

Original languageEnglish
Pages (from-to)14938-14953
Number of pages16
JournalACS Nano
Volume18
Issue number23
DOIs
Publication statusPublished - 11 Jun 2024

Keywords

  • advanced therapies
  • drug delivery system
  • gene therapy
  • medical devices
  • nanoneedles
  • porous silicon
  • topical delivery

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