Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection

Cong Wang; Chenlei Gu; Courtney Popp; Priya Vashisth; Salman Ahmad Mustfa; Davide Alessandro Martella; Chantelle Spiteri; Samuel McLennan; Ningjia Sun; Megan Riddle; Cindy R Eide; Maddy Parsons; Jakub Tolar; John A McGrath; Ciro Chiappini

doi:10.1021/acsnano.4c00206

Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection

Centre for Craniofacial and Regenerative Biology
King's College London
University of Minnesota
Randall Centre for Cell and Molecular Biophysics
St John's Institute of Dermatology

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell–nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.

Original language	English
Pages (from-to)	14938-14953
Number of pages	16
Journal	ACS Nano
Volume	18
Issue number	23
Early online date	10 May 2024
DOIs	https://doi.org/10.1021/acsnano.4c00206 https://doi.org/10.1021/acsnano.4c00206
Publication status	Published - 11 Jun 2024

Keywords

advanced therapies
drug delivery system
gene therapy
medical devices
nanoneedles
porous silicon
topical delivery

Access to Document

Cite this

Wang, C., Gu, C., Popp, C., Vashisth, P., Mustfa, S. A., Martella, D. A., Spiteri, C., McLennan, S., Sun, N., Riddle, M., Eide, C. R., Parsons, M., Tolar, J., McGrath, J. A., & Chiappini, C. (2024). Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection. ACS Nano, 18(23), 14938-14953. https://doi.org/10.1021/acsnano.4c00206, https://doi.org/10.1021/acsnano.4c00206

@article{99652d0026e34f83a5aff042bec3f6dc,

title = "Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection",

abstract = "Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell–nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.",

keywords = "advanced therapies, drug delivery system, gene therapy, medical devices, nanoneedles, porous silicon, topical delivery",

author = "Cong Wang and Chenlei Gu and Courtney Popp and Priya Vashisth and Mustfa, \{Salman Ahmad\} and Martella, \{Davide Alessandro\} and Chantelle Spiteri and Samuel McLennan and Ningjia Sun and Megan Riddle and Eide, \{Cindy R\} and Maddy Parsons and Jakub Tolar and McGrath, \{John A\} and Ciro Chiappini",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Published by American Chemical Society.",

year = "2024",

month = jun,

day = "11",

doi = "10.1021/acsnano.4c00206",

language = "English",

volume = "18",

pages = "14938--14953",

journal = "ACS Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "23",

}

Wang, C , Gu, C, Popp, C, Vashisth, P , Mustfa, SA , Martella, DA , Spiteri, C , McLennan, S , Sun, N, Riddle, M, Eide, CR, Parsons, M, Tolar, J, McGrath, JA & Chiappini, C 2024, 'Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection', ACS Nano, vol. 18, no. 23, pp. 14938-14953. https://doi.org/10.1021/acsnano.4c00206, https://doi.org/10.1021/acsnano.4c00206

TY - JOUR

T1 - Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection

AU - Wang, Cong

AU - Gu, Chenlei

AU - Popp, Courtney

AU - Vashisth, Priya

AU - Mustfa, Salman Ahmad

AU - Martella, Davide Alessandro

AU - Spiteri, Chantelle

AU - McLennan, Samuel

AU - Sun, Ningjia

AU - Riddle, Megan

AU - Eide, Cindy R

AU - Parsons, Maddy

AU - Tolar, Jakub

AU - McGrath, John A

AU - Chiappini, Ciro

PY - 2024/6/11

Y1 - 2024/6/11

N2 - Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell–nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.

AB - Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell–nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.

KW - advanced therapies

KW - drug delivery system

KW - gene therapy

KW - medical devices

KW - nanoneedles

KW - porous silicon

KW - topical delivery

UR - https://www.scopus.com/pages/publications/85192856203

U2 - 10.1021/acsnano.4c00206

DO - 10.1021/acsnano.4c00206

M3 - Article

C2 - 38726598

AN - SCOPUS:85192856203

SN - 1936-0851

VL - 18

SP - 14938

EP - 14953

JO - ACS Nano

JF - ACS Nano

IS - 23

ER -

Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this