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Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data

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Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data. / de Freitas, Marcela Medeiros; Souza, Paula Monteiro; Cruvinel, Kellen; Barros, Thais; Santos, Suikinai Nobre; Long, Paul F.; Pessoa, Adalberto; Magalhães, Pérola Oliveira.

In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Vol. 103, No. 13, 04.07.2019, p. 5161-5166.

Research output: Contribution to journalReview article

Harvard

de Freitas, MM, Souza, PM, Cruvinel, K, Barros, T, Santos, SN, Long, PF, Pessoa, A & Magalhães, PO 2019, 'Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data', APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 13, pp. 5161-5166. https://doi.org/10.1007/s00253-019-09890-0

APA

de Freitas, M. M., Souza, P. M., Cruvinel, K., Barros, T., Santos, S. N., Long, P. F., ... Magalhães, P. O. (2019). Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 103(13), 5161-5166. https://doi.org/10.1007/s00253-019-09890-0

Vancouver

de Freitas MM, Souza PM, Cruvinel K, Barros T, Santos SN, Long PF et al. Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2019 Jul 4;103(13):5161-5166. https://doi.org/10.1007/s00253-019-09890-0

Author

de Freitas, Marcela Medeiros ; Souza, Paula Monteiro ; Cruvinel, Kellen ; Barros, Thais ; Santos, Suikinai Nobre ; Long, Paul F. ; Pessoa, Adalberto ; Magalhães, Pérola Oliveira. / Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data. In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2019 ; Vol. 103, No. 13. pp. 5161-5166.

Bibtex Download

@article{0d7aae5a1a6341bf9534d22705232bff,
title = "Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data",
abstract = "l-asparaginase is an enzyme produced by microorganisms, plants, and animals, which is used clinically for the treatment for acute lymphoblastic leukemia (ALL) and, in the food industry, to control acrylamide formation in baked foods. The purpose of this review was to evaluate the available literature regarding microbial sources of l-asparaginase, culture media used to achieve maximum enzyme expression in microbial fermentations, and assay methods employed to assess l-asparaginase activity. Studies were gathered by searching PubMed, and Web of Science databases before January 22, 2018, with no time restrictions. The articles were evaluated according to the source of l-asparaginase being studied, the nitrogen source in the culture medium, the type of sample, and the method employed to evaluate l-asparaginase activity. Bacterial l-asparaginase appeared to be the most commonly studied source of the enzyme and, most often, the enzyme activity was assayed from crude protein extracts using the Nessler method, which is an indirect measurement of asparaginase activity that determines the concentration of ammonia generated after the action of the enzyme on the substrate, l-asparagine. However, ammonia is also generated throughout microbial fermentations and this endogenous ammonia will also reduce the Nessler reagent if crude microbial extracts are used to determine total l-asparaginase activity. We suggest that current estimates of l-asparaginase activity reported in the literature may be overestimated when Nessler reagent is used, since we were unable to find a single study that made reference to the possible inference of fermentation derived ammonia.",
keywords = "Enzyme assay, Interferences, l-Asparaginase, Nessler method",
author = "{de Freitas}, {Marcela Medeiros} and Souza, {Paula Monteiro} and Kellen Cruvinel and Thais Barros and Santos, {Suikinai Nobre} and Long, {Paul F.} and Adalberto Pessoa and Magalh{\~a}es, {P{\'e}rola Oliveira}",
year = "2019",
month = "7",
day = "4",
doi = "10.1007/s00253-019-09890-0",
language = "English",
volume = "103",
pages = "5161--5166",
journal = "APPLIED MICROBIOLOGY AND BIOTECHNOLOGY",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "13",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Interferences that impact measuring optimal l-asparaginase activity and consequent errors interpreting these data

AU - de Freitas, Marcela Medeiros

AU - Souza, Paula Monteiro

AU - Cruvinel, Kellen

AU - Barros, Thais

AU - Santos, Suikinai Nobre

AU - Long, Paul F.

AU - Pessoa, Adalberto

AU - Magalhães, Pérola Oliveira

PY - 2019/7/4

Y1 - 2019/7/4

N2 - l-asparaginase is an enzyme produced by microorganisms, plants, and animals, which is used clinically for the treatment for acute lymphoblastic leukemia (ALL) and, in the food industry, to control acrylamide formation in baked foods. The purpose of this review was to evaluate the available literature regarding microbial sources of l-asparaginase, culture media used to achieve maximum enzyme expression in microbial fermentations, and assay methods employed to assess l-asparaginase activity. Studies were gathered by searching PubMed, and Web of Science databases before January 22, 2018, with no time restrictions. The articles were evaluated according to the source of l-asparaginase being studied, the nitrogen source in the culture medium, the type of sample, and the method employed to evaluate l-asparaginase activity. Bacterial l-asparaginase appeared to be the most commonly studied source of the enzyme and, most often, the enzyme activity was assayed from crude protein extracts using the Nessler method, which is an indirect measurement of asparaginase activity that determines the concentration of ammonia generated after the action of the enzyme on the substrate, l-asparagine. However, ammonia is also generated throughout microbial fermentations and this endogenous ammonia will also reduce the Nessler reagent if crude microbial extracts are used to determine total l-asparaginase activity. We suggest that current estimates of l-asparaginase activity reported in the literature may be overestimated when Nessler reagent is used, since we were unable to find a single study that made reference to the possible inference of fermentation derived ammonia.

AB - l-asparaginase is an enzyme produced by microorganisms, plants, and animals, which is used clinically for the treatment for acute lymphoblastic leukemia (ALL) and, in the food industry, to control acrylamide formation in baked foods. The purpose of this review was to evaluate the available literature regarding microbial sources of l-asparaginase, culture media used to achieve maximum enzyme expression in microbial fermentations, and assay methods employed to assess l-asparaginase activity. Studies were gathered by searching PubMed, and Web of Science databases before January 22, 2018, with no time restrictions. The articles were evaluated according to the source of l-asparaginase being studied, the nitrogen source in the culture medium, the type of sample, and the method employed to evaluate l-asparaginase activity. Bacterial l-asparaginase appeared to be the most commonly studied source of the enzyme and, most often, the enzyme activity was assayed from crude protein extracts using the Nessler method, which is an indirect measurement of asparaginase activity that determines the concentration of ammonia generated after the action of the enzyme on the substrate, l-asparagine. However, ammonia is also generated throughout microbial fermentations and this endogenous ammonia will also reduce the Nessler reagent if crude microbial extracts are used to determine total l-asparaginase activity. We suggest that current estimates of l-asparaginase activity reported in the literature may be overestimated when Nessler reagent is used, since we were unable to find a single study that made reference to the possible inference of fermentation derived ammonia.

KW - Enzyme assay

KW - Interferences

KW - l-Asparaginase

KW - Nessler method

UR - http://www.scopus.com/inward/record.url?scp=85066088947&partnerID=8YFLogxK

U2 - 10.1007/s00253-019-09890-0

DO - 10.1007/s00253-019-09890-0

M3 - Review article

C2 - 31104099

AN - SCOPUS:85066088947

VL - 103

SP - 5161

EP - 5166

JO - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

JF - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

SN - 0175-7598

IS - 13

ER -

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