Interferon-alpha 2a is sufficient for promoting dendritic cell immunogenicity

A Tamir, W J Jordan, M Ritter, N Habib, R I Lechler, G R Foster, G Lombardi

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    15 Citations (Scopus)

    Abstract

    Type I interferons (IFNs) are widely used therapeutically. IFN-alpha 2a in particular is used as an antiviral agent, but its immunomodulatory properties are poorly understood. Dendritic cells (DCs) are the only antigen-presenting cells able to prime naive T cells and therefore play a crucial role in initiating the adaptive phase of the immune response. We studied the effects of IFN-alpha 2a on DC maturation and its role in determining Th1/Th2 equilibrium. We found that IFN-alpha 2a induced phenotypic maturation of DCs and increased their allo-stimulatory capacity. When dendritic cells were stimulated simultaneously by CD40 ligation and IFN-alpha 2a, the production of interleukin (IL)-10 and IL-12 was increased. In contrast, lipopolysaccharide (LPS) stimulation in the presence of IFN-alpha 2a mainly induced IL-10 release. The production of IFN-gamma and IL-5 by the responder naive T cells was also amplified in response to IFN-alpha 2a-treated DCs. Furthermore, IL-12 production by IFN-alpha 2a-treated DCs was enhanced further in the presence of anti-IL-10 antibody. Different results were obtained when DCs were treated simultaneously with IFN-alpha 2a and other maturation factors, in particular LPS, and then stimulated by CD40 ligation 36 h later. Under these circumstances, IFN-alpha 2a did not modify the DC phenotype, and the production of IL-10/IL-12 and IFN-gamma/IL-5 by DCs and by DC-stimulated naive T cells, respectively, was inhibited compared to the effects on DCs treated with maturation factors alone. Altogether, this work suggests that IFN-alpha 2a in isolation is sufficient to promote DC activation, however, other concomitant events, such as exposure to LPS during a bacterial infection, can inhibit its effects. These results clarify some of the in vivo findings obtained with IFN-alpha 2a and have direct implications for the design of IFN-alpha-based vaccines for immunotherapy
    Original languageEnglish
    Pages (from-to)471 - 480
    Number of pages10
    JournalClinical and Experimental Immunology
    Volume142
    Issue number3
    DOIs
    Publication statusPublished - Dec 2005

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