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Intestinal virome in patients with alcoholic hepatitis

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Intestinal virome in patients with alcoholic hepatitis. / Jiang, Lu; Lang, Sonja; Duan, Yi; Zhang, Xinlian; Gao, Bei; Chopyk, Jessica; Schwanemann, Leila K; Ventura-Cots, Meritxell; Bataller, Ramon; Bosques-Padilla, Francisco; Verna, Elizabeth C; Abraldes, Juan G; Brown, Robert S; Vargas, Victor; Altamirano, Jose; Caballería, Juan; Shawcross, Debbie L; Ho, Samuel B; Louvet, Alexandre; Lucey, Michael R; Mathurin, Philippe; Garcia-Tsao, Guadalupe; Kisseleva, Tatiana; Brenner, David A; Tu, Xin M; Stärkel, Peter; Pride, David; Fouts, Derrick E; Schnabl, Bernd.

In: Hepatology, 12.07.2020.

Research output: Contribution to journalArticle

Harvard

Jiang, L, Lang, S, Duan, Y, Zhang, X, Gao, B, Chopyk, J, Schwanemann, LK, Ventura-Cots, M, Bataller, R, Bosques-Padilla, F, Verna, EC, Abraldes, JG, Brown, RS, Vargas, V, Altamirano, J, Caballería, J, Shawcross, DL, Ho, SB, Louvet, A, Lucey, MR, Mathurin, P, Garcia-Tsao, G, Kisseleva, T, Brenner, DA, Tu, XM, Stärkel, P, Pride, D, Fouts, DE & Schnabl, B 2020, 'Intestinal virome in patients with alcoholic hepatitis', Hepatology. https://doi.org/10.1002/hep.31459

APA

Jiang, L., Lang, S., Duan, Y., Zhang, X., Gao, B., Chopyk, J., Schwanemann, L. K., Ventura-Cots, M., Bataller, R., Bosques-Padilla, F., Verna, E. C., Abraldes, J. G., Brown, R. S., Vargas, V., Altamirano, J., Caballería, J., Shawcross, D. L., Ho, S. B., Louvet, A., ... Schnabl, B. (2020). Intestinal virome in patients with alcoholic hepatitis. Hepatology. https://doi.org/10.1002/hep.31459

Vancouver

Jiang L, Lang S, Duan Y, Zhang X, Gao B, Chopyk J et al. Intestinal virome in patients with alcoholic hepatitis. Hepatology. 2020 Jul 12. https://doi.org/10.1002/hep.31459

Author

Jiang, Lu ; Lang, Sonja ; Duan, Yi ; Zhang, Xinlian ; Gao, Bei ; Chopyk, Jessica ; Schwanemann, Leila K ; Ventura-Cots, Meritxell ; Bataller, Ramon ; Bosques-Padilla, Francisco ; Verna, Elizabeth C ; Abraldes, Juan G ; Brown, Robert S ; Vargas, Victor ; Altamirano, Jose ; Caballería, Juan ; Shawcross, Debbie L ; Ho, Samuel B ; Louvet, Alexandre ; Lucey, Michael R ; Mathurin, Philippe ; Garcia-Tsao, Guadalupe ; Kisseleva, Tatiana ; Brenner, David A ; Tu, Xin M ; Stärkel, Peter ; Pride, David ; Fouts, Derrick E ; Schnabl, Bernd. / Intestinal virome in patients with alcoholic hepatitis. In: Hepatology. 2020.

Bibtex Download

@article{387b5bd6e2bf450b86fcad6827b5c808,
title = "Intestinal virome in patients with alcoholic hepatitis",
abstract = "Background and Aims: Alcoholic hepatitis (AH) is a severe manifestation of alcohol-associated liver disease (ALD) with high mortality. Although gut bacteria and fungi modulate disease severity, little is known about the effects of the viral microbiome (virome) in patients with ALD. Approach and Results: We extracted virus-like particles from 89 patients with AH who were enrolled in a multicenter observational study, 36 with alcohol use disorder (AUD), and 17 persons without AUD (controls). Virus-like particles from fecal samples were fractionated using differential filtration techniques, and metagenomic sequencing was performed to characterize intestinal viromes. We observed an increased viral diversity in fecal samples from patients with ALD, with the most significant changes in samples from patients with AH. Escherichia-, Enterobacteria-, and Enterococcus phages were over-represented in fecal samples from patients with AH, along with significant increases in mammalian viruses such as Parvoviridae and Herpesviridae. Antibiotic treatment was associated with higher viral diversity. Specific viral taxa, such as Staphylococcus phages and Herpesviridae, were associated with increased disease severity, indicated by a higher median Model for End-Stage Liver Disease score, and associated with increased 90-day mortality. Conclusions: In conclusion, intestinal viral taxa are altered in fecal samples from patients with AH and associated with disease severity and mortality. Our study describes an intestinal virome signature associated with AH.",
author = "Lu Jiang and Sonja Lang and Yi Duan and Xinlian Zhang and Bei Gao and Jessica Chopyk and Schwanemann, {Leila K} and Meritxell Ventura-Cots and Ramon Bataller and Francisco Bosques-Padilla and Verna, {Elizabeth C} and Abraldes, {Juan G} and Brown, {Robert S} and Victor Vargas and Jose Altamirano and Juan Caballer{\'i}a and Shawcross, {Debbie L} and Ho, {Samuel B} and Alexandre Louvet and Lucey, {Michael R} and Philippe Mathurin and Guadalupe Garcia-Tsao and Tatiana Kisseleva and Brenner, {David A} and Tu, {Xin M} and Peter St{\"a}rkel and David Pride and Fouts, {Derrick E} and Bernd Schnabl",
note = "This article is protected by copyright. All rights reserved.",
year = "2020",
month = jul,
day = "12",
doi = "10.1002/hep.31459",
language = "English",
journal = "Hepatology",
issn = "0270-9139",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Intestinal virome in patients with alcoholic hepatitis

AU - Jiang, Lu

AU - Lang, Sonja

AU - Duan, Yi

AU - Zhang, Xinlian

AU - Gao, Bei

AU - Chopyk, Jessica

AU - Schwanemann, Leila K

AU - Ventura-Cots, Meritxell

AU - Bataller, Ramon

AU - Bosques-Padilla, Francisco

AU - Verna, Elizabeth C

AU - Abraldes, Juan G

AU - Brown, Robert S

AU - Vargas, Victor

AU - Altamirano, Jose

AU - Caballería, Juan

AU - Shawcross, Debbie L

AU - Ho, Samuel B

AU - Louvet, Alexandre

AU - Lucey, Michael R

AU - Mathurin, Philippe

AU - Garcia-Tsao, Guadalupe

AU - Kisseleva, Tatiana

AU - Brenner, David A

AU - Tu, Xin M

AU - Stärkel, Peter

AU - Pride, David

AU - Fouts, Derrick E

AU - Schnabl, Bernd

N1 - This article is protected by copyright. All rights reserved.

PY - 2020/7/12

Y1 - 2020/7/12

N2 - Background and Aims: Alcoholic hepatitis (AH) is a severe manifestation of alcohol-associated liver disease (ALD) with high mortality. Although gut bacteria and fungi modulate disease severity, little is known about the effects of the viral microbiome (virome) in patients with ALD. Approach and Results: We extracted virus-like particles from 89 patients with AH who were enrolled in a multicenter observational study, 36 with alcohol use disorder (AUD), and 17 persons without AUD (controls). Virus-like particles from fecal samples were fractionated using differential filtration techniques, and metagenomic sequencing was performed to characterize intestinal viromes. We observed an increased viral diversity in fecal samples from patients with ALD, with the most significant changes in samples from patients with AH. Escherichia-, Enterobacteria-, and Enterococcus phages were over-represented in fecal samples from patients with AH, along with significant increases in mammalian viruses such as Parvoviridae and Herpesviridae. Antibiotic treatment was associated with higher viral diversity. Specific viral taxa, such as Staphylococcus phages and Herpesviridae, were associated with increased disease severity, indicated by a higher median Model for End-Stage Liver Disease score, and associated with increased 90-day mortality. Conclusions: In conclusion, intestinal viral taxa are altered in fecal samples from patients with AH and associated with disease severity and mortality. Our study describes an intestinal virome signature associated with AH.

AB - Background and Aims: Alcoholic hepatitis (AH) is a severe manifestation of alcohol-associated liver disease (ALD) with high mortality. Although gut bacteria and fungi modulate disease severity, little is known about the effects of the viral microbiome (virome) in patients with ALD. Approach and Results: We extracted virus-like particles from 89 patients with AH who were enrolled in a multicenter observational study, 36 with alcohol use disorder (AUD), and 17 persons without AUD (controls). Virus-like particles from fecal samples were fractionated using differential filtration techniques, and metagenomic sequencing was performed to characterize intestinal viromes. We observed an increased viral diversity in fecal samples from patients with ALD, with the most significant changes in samples from patients with AH. Escherichia-, Enterobacteria-, and Enterococcus phages were over-represented in fecal samples from patients with AH, along with significant increases in mammalian viruses such as Parvoviridae and Herpesviridae. Antibiotic treatment was associated with higher viral diversity. Specific viral taxa, such as Staphylococcus phages and Herpesviridae, were associated with increased disease severity, indicated by a higher median Model for End-Stage Liver Disease score, and associated with increased 90-day mortality. Conclusions: In conclusion, intestinal viral taxa are altered in fecal samples from patients with AH and associated with disease severity and mortality. Our study describes an intestinal virome signature associated with AH.

UR - http://www.scopus.com/inward/record.url?scp=85092328654&partnerID=8YFLogxK

U2 - 10.1002/hep.31459

DO - 10.1002/hep.31459

M3 - Article

C2 - 32654263

JO - Hepatology

JF - Hepatology

SN - 0270-9139

ER -

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