Intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other enterobacteria

A Verma; N Desai; K Shannon; J Philpott-Howard; R L R Hill

doi:10.1016/S0924-8579(00)00324-1

Intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other enterobacteria

A Verma, N Desai, K Shannon, J Philpott-Howard, R L R Hill

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

klebsiella aerogenes K41, resistant to third generation cephalosporins and aminoglycosides, was isolated From clinical samples of 153 in-patients. Blood cultures accounted for 24 (15.7%) of isolates. The MIG,,, of ceftazidime for thr isolates of 84 patients was >512 mg/l and was reduced to 2.0 by 4 mg/l of clavulanic acid, but only to 64 by 4 mg/l of sulbactam. Isolates of K. aerogenes K41 produced extended-spectrum beta -lactamase (ESBL) SHV-5 and TEM-1, identified by isoelectric focusing. Plasmid profiles showed that co-dissemination of cephalosporin and aminoglycoside resistance, plus ESBL production, coincided with the acquisition of a 116-kb plasmid. This plasmid was transferable in vitro from K. aerogenes K41 to other serotypes and genera of the Enterobacteriaceae. (C) 2001 Published by Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.

Original language	English
Pages (from-to)	123 - 129
Number of pages	7
Journal	INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume	17
Issue number	2
DOIs	https://doi.org/10.1016/S0924-8579(00)00324-1
Publication status	Published - 2001

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title = "Intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other enterobacteria",

abstract = "klebsiella aerogenes K41, resistant to third generation cephalosporins and aminoglycosides, was isolated From clinical samples of 153 in-patients. Blood cultures accounted for 24 (15.7%) of isolates. The MIG,,, of ceftazidime for thr isolates of 84 patients was >512 mg/l and was reduced to 2.0 by 4 mg/l of clavulanic acid, but only to 64 by 4 mg/l of sulbactam. Isolates of K. aerogenes K41 produced extended-spectrum beta -lactamase (ESBL) SHV-5 and TEM-1, identified by isoelectric focusing. Plasmid profiles showed that co-dissemination of cephalosporin and aminoglycoside resistance, plus ESBL production, coincided with the acquisition of a 116-kb plasmid. This plasmid was transferable in vitro from K. aerogenes K41 to other serotypes and genera of the Enterobacteriaceae. (C) 2001 Published by Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.",

author = "A Verma and N Desai and K Shannon and J Philpott-Howard and Hill, {R L R}",

year = "2001",

doi = "10.1016/S0924-8579(00)00324-1",

language = "English",

volume = "17",

pages = "123 -- 129",

journal = "INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS",

publisher = "Elsevier",

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T1 - Intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other enterobacteria

AU - Verma, A

AU - Desai, N

AU - Shannon, K

AU - Philpott-Howard, J

AU - Hill, R L R

PY - 2001

Y1 - 2001

N2 - klebsiella aerogenes K41, resistant to third generation cephalosporins and aminoglycosides, was isolated From clinical samples of 153 in-patients. Blood cultures accounted for 24 (15.7%) of isolates. The MIG,,, of ceftazidime for thr isolates of 84 patients was >512 mg/l and was reduced to 2.0 by 4 mg/l of clavulanic acid, but only to 64 by 4 mg/l of sulbactam. Isolates of K. aerogenes K41 produced extended-spectrum beta -lactamase (ESBL) SHV-5 and TEM-1, identified by isoelectric focusing. Plasmid profiles showed that co-dissemination of cephalosporin and aminoglycoside resistance, plus ESBL production, coincided with the acquisition of a 116-kb plasmid. This plasmid was transferable in vitro from K. aerogenes K41 to other serotypes and genera of the Enterobacteriaceae. (C) 2001 Published by Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.

AB - klebsiella aerogenes K41, resistant to third generation cephalosporins and aminoglycosides, was isolated From clinical samples of 153 in-patients. Blood cultures accounted for 24 (15.7%) of isolates. The MIG,,, of ceftazidime for thr isolates of 84 patients was >512 mg/l and was reduced to 2.0 by 4 mg/l of clavulanic acid, but only to 64 by 4 mg/l of sulbactam. Isolates of K. aerogenes K41 produced extended-spectrum beta -lactamase (ESBL) SHV-5 and TEM-1, identified by isoelectric focusing. Plasmid profiles showed that co-dissemination of cephalosporin and aminoglycoside resistance, plus ESBL production, coincided with the acquisition of a 116-kb plasmid. This plasmid was transferable in vitro from K. aerogenes K41 to other serotypes and genera of the Enterobacteriaceae. (C) 2001 Published by Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.

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U2 - 10.1016/S0924-8579(00)00324-1

DO - 10.1016/S0924-8579(00)00324-1

M3 - Article

VL - 17

SP - 123

EP - 129

JO - INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

JF - INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

IS - 2

ER -

Intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other enterobacteria

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