TY - JOUR
T1 - Intracellular calcium handling in rat olfactory ensheathing cells and its role in axonal regeneration
AU - Hayat, S
AU - Wigley, C B
AU - Robbins, J
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Intracellular calcium handling by rat olfactory ensheathing cells (OECs) is implicated in their support for regrowth of adult CNS neurites in a coculture model of axonal regeneration. Pretreatment of OECs with BAPTA-AM to sequester glial intracellular calcium ([Ca2+](i)) reduces significantly the numbers of cocultured neurons regrowing neurites. The mean resting [Ca2+](i) of OECs cultured alone or with neurons was 300 nM in an external solution containing 2.5 mM calcium ([Ca2+](o)). In high [K+](o) or zero [Ca2+](o), resting [Ca2+](i) significantly decreased. [Ca2+](i) significantly increased when [Ca2+](o) was increased to 20 mM, lonomycin, thapsigargin, and thimerosal increased [Ca2+](i), and caffeine, ryanodine, and cyclopiazonic acid were without effect. Of the receptor agonists tested, none induced a change in [Ca2+](i). The calcium influx induced by high [Ca2+](o) was blocked by La3+ and SKF96365, partially inhibited by Cd2+, and insensitive to Ni2+ and nifedipine. Pretreatment of OECs with La3+ reduced neurite regrowth in cocultures in a concentration-dependent manner over the range that blocked the non-voltage-gated calcium flux through a putative TRP-like channel, which, we propose, is activated in OEC-mediated axonal regeneration. (C) 2003 Elsevier Science (USA). All rights reserved.
AB - Intracellular calcium handling by rat olfactory ensheathing cells (OECs) is implicated in their support for regrowth of adult CNS neurites in a coculture model of axonal regeneration. Pretreatment of OECs with BAPTA-AM to sequester glial intracellular calcium ([Ca2+](i)) reduces significantly the numbers of cocultured neurons regrowing neurites. The mean resting [Ca2+](i) of OECs cultured alone or with neurons was 300 nM in an external solution containing 2.5 mM calcium ([Ca2+](o)). In high [K+](o) or zero [Ca2+](o), resting [Ca2+](i) significantly decreased. [Ca2+](i) significantly increased when [Ca2+](o) was increased to 20 mM, lonomycin, thapsigargin, and thimerosal increased [Ca2+](i), and caffeine, ryanodine, and cyclopiazonic acid were without effect. Of the receptor agonists tested, none induced a change in [Ca2+](i). The calcium influx induced by high [Ca2+](o) was blocked by La3+ and SKF96365, partially inhibited by Cd2+, and insensitive to Ni2+ and nifedipine. Pretreatment of OECs with La3+ reduced neurite regrowth in cocultures in a concentration-dependent manner over the range that blocked the non-voltage-gated calcium flux through a putative TRP-like channel, which, we propose, is activated in OEC-mediated axonal regeneration. (C) 2003 Elsevier Science (USA). All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=0037301343&partnerID=8YFLogxK
U2 - 10.1016/S1044-7431(03)00051-4
DO - 10.1016/S1044-7431(03)00051-4
M3 - Article
VL - 22
SP - 259
EP - 270
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
IS - 2
ER -