Intrahepatic cholestasis of pregnancy levels of sulfated progesterone metabolites inhibit farnesoid X receptor resulting in a cholestatic phenotype

Shadi Abu-Hayyeh, Georgia Papacleovoulou, Anita Lövgren-Sandblom, Mehreen Tahir, Olayiwola Oduwole, Nurul Akmal Jamaludin, Sabiha Ravat, Vanya Nikolova, Jenny Chambers, Clare Selden, Myrddin Rees, Hanns-Ulrich Marschall, Malcolm G Parker, Catherine Williamson

Research output: Contribution to journalArticlepeer-review

133 Citations (Scopus)


Intrahepatic cholestasis of pregnancy (ICP) is the most prevalent pregnancy-specific liver disease and is associated with an increased risk of adverse fetal outcomes, including preterm labor and intrauterine death. The endocrine signals that cause cholestasis are not known but 3α-sulfated progesterone metabolites have been shown to be elevated in ICP, leading us to study the impact of sulfated progesterone metabolites on farnesoid X receptor (FXR)-mediated bile acid homeostasis pathways. Here we report that the 3β-sulfated progesterone metabolite epiallopregnanolone sulfate is supraphysiologically raised in the serum of ICP patients. Mice challenged with cholic acid developed hypercholanemia and a hepatic gene expression profile indicative of FXR activation. However, coadministration of epiallopregnanolone sulfate with cholic acid exacerbated the hypercholanemia and resulted in aberrant gene expression profiles for hepatic bile acid-responsive genes consistent with cholestasis. We demonstrate that levels of epiallopregnanolone sulfate found in ICP can function as a partial agonist for FXR, resulting in the aberrant expression of bile acid homeostasis genes in hepatoma cell lines and primary human hepatocytes. Furthermore, epiallopregnanolone sulfate inhibition of FXR results in reduced FXR-mediated bile acid efflux and secreted FGF19. Using cofactor recruitment assays, we show that epiallopregnanolone sulfate competitively inhibits bile acid-mediated recruitment of cofactor motifs to the FXR-ligand binding domain. CONCLUSION: Our results reveal a novel molecular interaction between ICP-associated levels of the 3β-sulfated progesterone metabolite epiallopregnanolone sulfate and FXR that couples the endocrine component of pregnancy in ICP to abnormal bile acid homeostasis.
Original languageEnglish
Pages (from-to)716-726
Number of pages11
Issue number2
Publication statusPublished - Feb 2013


  • Receptors, Cytoplasmic and Nuclear
  • Animals
  • Cholic Acid
  • Pregnancy Complications
  • Progesterone
  • Cholestasis
  • Humans
  • Mice
  • Homeostasis
  • Pregnancy
  • Sulfuric Acid Esters
  • Phenotype
  • Pregnanolone
  • Cholestasis, Intrahepatic
  • Bile Acids and Salts
  • Mice, Inbred C57BL
  • Female


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