Introduction to cell culture

TY - JOUR

T1 - Introduction to cell culture

AU - Philippeos, Christina

AU - Hughes, Robin D

AU - Dhawan, Anil

AU - Mitry, Ragai R

PY - 2012

Y1 - 2012

N2 - The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture. Steps must be taken to maintain aseptic conditions to prevent contamination of cultures with micro-organisms. Basic cell-handling techniques are discussed, including choice of media, primary culture, and cryopreservation of cells so they can be stored for future use. Common assays which are used to determine cell viability and activity are considered.

AB - The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture. Steps must be taken to maintain aseptic conditions to prevent contamination of cultures with micro-organisms. Basic cell-handling techniques are discussed, including choice of media, primary culture, and cryopreservation of cells so they can be stored for future use. Common assays which are used to determine cell viability and activity are considered.

KW - Animals

KW - Cell Culture Techniques

KW - Humans

KW - Laboratories

KW - Journal Article

U2 - 10.1007/978-1-61779-367-7_1

DO - 10.1007/978-1-61779-367-7_1

M3 - Article

C2 - 22057441

SN - 1940-6029

VL - 806

SP - 1

EP - 13

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -