Investigating the Potential of Cuboidal Nanometals as Protein Subunit Vaccine Carriers In Vivo

Emine Yavuz; Adam A. Walters; Bhavnesh V. Chudasama; Shunping Han; Yue Qin; Khuloud T. Al-Jamal

doi:10.1002/admi.202202511

Investigating the Potential of Cuboidal Nanometals as Protein Subunit Vaccine Carriers In Vivo

Emine Yavuz, Adam A. Walters, Bhavnesh V. Chudasama, Shunping Han, Yue Qin, Khuloud T. Al-Jamal^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Metal nanoparticles (NPs) are suggested as a vaccine delivery platform. At present, there is limited description of cuboidal Ag nanocubes (AgNCs; nonporous) and Au nanocages (AuNCs; porous) as a protein carrier for vaccination. Here, the intrinsic protein binding ability of AgNC and AuNC is first investigated, using ovalbumin (OVA) as a model antigen, to determine its suitability as a vaccine carrier. Next, the effect of AgNCs and AuNCs on bone-marrow-derived dendritic cells (BMDCs) is assessed in vitro. Finally, in vivo humoral and cellular immune responses of AgNC–OVA and AuNC–OVA following intramuscular immunization and their prophylactic effects in B16F10-OVA mice tumor model are investigated. In terms of OVA loading efficiency, AgNCs are superior to AuNCs. Both nanomaterials are found not to induce BMDC maturation at subtoxic doses. After administration of nanovaccines, serum immunoglobulin G (IgG) responses are comparable between groups. However, there are slight alterations in relative frequencies of lymphocyte subpopulations, with AgNC–OVA-immunized mice exhibiting lower memory T cells and reduced B cell and T follicular helper cell populations in spleen. Overall, AgNC–OVA and AuNC–OVA immunizations do not alter tumor growth. This study characterizes the intrinsic immunomodulatory properties of AgNCs and AuNCs, as protein subunit vaccine carriers.

Original language	English
Article number	2202511
Journal	advanced materials interfaces
Volume	10
Issue number	29
DOIs	https://doi.org/10.1002/admi.202202511
Publication status	Published - 13 Oct 2023

Keywords

cancer vaccine
gold nanocages
melanoma
nanocarriers
silver nanocubes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/admi.202202511

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title = "Investigating the Potential of Cuboidal Nanometals as Protein Subunit Vaccine Carriers In Vivo",

abstract = "Metal nanoparticles (NPs) are suggested as a vaccine delivery platform. At present, there is limited description of cuboidal Ag nanocubes (AgNCs; nonporous) and Au nanocages (AuNCs; porous) as a protein carrier for vaccination. Here, the intrinsic protein binding ability of AgNC and AuNC is first investigated, using ovalbumin (OVA) as a model antigen, to determine its suitability as a vaccine carrier. Next, the effect of AgNCs and AuNCs on bone-marrow-derived dendritic cells (BMDCs) is assessed in vitro. Finally, in vivo humoral and cellular immune responses of AgNC–OVA and AuNC–OVA following intramuscular immunization and their prophylactic effects in B16F10-OVA mice tumor model are investigated. In terms of OVA loading efficiency, AgNCs are superior to AuNCs. Both nanomaterials are found not to induce BMDC maturation at subtoxic doses. After administration of nanovaccines, serum immunoglobulin G (IgG) responses are comparable between groups. However, there are slight alterations in relative frequencies of lymphocyte subpopulations, with AgNC–OVA-immunized mice exhibiting lower memory T cells and reduced B cell and T follicular helper cell populations in spleen. Overall, AgNC–OVA and AuNC–OVA immunizations do not alter tumor growth. This study characterizes the intrinsic immunomodulatory properties of AgNCs and AuNCs, as protein subunit vaccine carriers.",

keywords = "cancer vaccine, gold nanocages, melanoma, nanocarriers, silver nanocubes",

author = "Emine Yavuz and Walters, {Adam A.} and Chudasama, {Bhavnesh V.} and Shunping Han and Yue Qin and Al-Jamal, {Khuloud T.}",

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AU - Han, Shunping

AU - Qin, Yue

AU - Al-Jamal, Khuloud T.

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AB - Metal nanoparticles (NPs) are suggested as a vaccine delivery platform. At present, there is limited description of cuboidal Ag nanocubes (AgNCs; nonporous) and Au nanocages (AuNCs; porous) as a protein carrier for vaccination. Here, the intrinsic protein binding ability of AgNC and AuNC is first investigated, using ovalbumin (OVA) as a model antigen, to determine its suitability as a vaccine carrier. Next, the effect of AgNCs and AuNCs on bone-marrow-derived dendritic cells (BMDCs) is assessed in vitro. Finally, in vivo humoral and cellular immune responses of AgNC–OVA and AuNC–OVA following intramuscular immunization and their prophylactic effects in B16F10-OVA mice tumor model are investigated. In terms of OVA loading efficiency, AgNCs are superior to AuNCs. Both nanomaterials are found not to induce BMDC maturation at subtoxic doses. After administration of nanovaccines, serum immunoglobulin G (IgG) responses are comparable between groups. However, there are slight alterations in relative frequencies of lymphocyte subpopulations, with AgNC–OVA-immunized mice exhibiting lower memory T cells and reduced B cell and T follicular helper cell populations in spleen. Overall, AgNC–OVA and AuNC–OVA immunizations do not alter tumor growth. This study characterizes the intrinsic immunomodulatory properties of AgNCs and AuNCs, as protein subunit vaccine carriers.

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Investigating the Potential of Cuboidal Nanometals as Protein Subunit Vaccine Carriers In Vivo

Abstract

Keywords

UN SDGs

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