Investigation of podosome ring protein arrangement using localization microscopy images

Adela D. Staszowska; Patrick Fox-Roberts; Elizabeth Foxall; Gareth E. Jones; Susan Cox

doi:10.1016/j.ymeth.2016.11.005

Investigation of podosome ring protein arrangement using localization microscopy images

Adela D. Staszowska, Patrick Fox-Roberts, Elizabeth Foxall, Gareth E. Jones, Susan Cox^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

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Abstract

Podosomes are adhesive structures formed on the plasma membrane abutting the extracellular matrix of macrophages, osteoclasts, and dendritic cells. They consist of an f-actin core and a ring structure composed of integrins and integrin-associated proteins. The podosome ring plays a major role in adhesion to the underlying extracellular matrix, but its detailed structure is poorly understood. Recently, it has become possible to study the nano-scale structure of podosome rings using localization microscopy. Unlike traditional microscopy images, localization microscopy images are reconstructed using discrete points, meaning that standard image analysis methods cannot be applied. Here, we present a pipeline for podosome identification, protein position calculation, and creating a podosome ring model for use with localization microscopy data.

Original language	English
Pages (from-to)	9-16
Journal	Methods
Volume	115
Early online date	10 Nov 2016
DOIs	https://doi.org/10.1016/j.ymeth.2016.11.005
Publication status	Published - Feb 2017

Keywords

Image analysis
Localization microscopy
Pattern recognition
Podosomes
Super-resolution microscopy

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10.1016/j.ymeth.2016.11.005Licence: CC BY

Investigation of podosome_STASZOWSKA_Accepted7November2016_GOLD VoR (CC BY)Final published version, 1.75 MBLicence: CC BY

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title = "Investigation of podosome ring protein arrangement using localization microscopy images",

abstract = "Podosomes are adhesive structures formed on the plasma membrane abutting the extracellular matrix of macrophages, osteoclasts, and dendritic cells. They consist of an f-actin core and a ring structure composed of integrins and integrin-associated proteins. The podosome ring plays a major role in adhesion to the underlying extracellular matrix, but its detailed structure is poorly understood. Recently, it has become possible to study the nano-scale structure of podosome rings using localization microscopy. Unlike traditional microscopy images, localization microscopy images are reconstructed using discrete points, meaning that standard image analysis methods cannot be applied. Here, we present a pipeline for podosome identification, protein position calculation, and creating a podosome ring model for use with localization microscopy data.",

keywords = "Image analysis, Localization microscopy, Pattern recognition, Podosomes, Super-resolution microscopy",

author = "Staszowska, {Adela D.} and Patrick Fox-Roberts and Elizabeth Foxall and Jones, {Gareth E.} and Susan Cox",

year = "2017",

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journal = "Methods",

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AU - Staszowska, Adela D.

AU - Fox-Roberts, Patrick

AU - Foxall, Elizabeth

AU - Jones, Gareth E.

AU - Cox, Susan

PY - 2017/2

Y1 - 2017/2

N2 - Podosomes are adhesive structures formed on the plasma membrane abutting the extracellular matrix of macrophages, osteoclasts, and dendritic cells. They consist of an f-actin core and a ring structure composed of integrins and integrin-associated proteins. The podosome ring plays a major role in adhesion to the underlying extracellular matrix, but its detailed structure is poorly understood. Recently, it has become possible to study the nano-scale structure of podosome rings using localization microscopy. Unlike traditional microscopy images, localization microscopy images are reconstructed using discrete points, meaning that standard image analysis methods cannot be applied. Here, we present a pipeline for podosome identification, protein position calculation, and creating a podosome ring model for use with localization microscopy data.

AB - Podosomes are adhesive structures formed on the plasma membrane abutting the extracellular matrix of macrophages, osteoclasts, and dendritic cells. They consist of an f-actin core and a ring structure composed of integrins and integrin-associated proteins. The podosome ring plays a major role in adhesion to the underlying extracellular matrix, but its detailed structure is poorly understood. Recently, it has become possible to study the nano-scale structure of podosome rings using localization microscopy. Unlike traditional microscopy images, localization microscopy images are reconstructed using discrete points, meaning that standard image analysis methods cannot be applied. Here, we present a pipeline for podosome identification, protein position calculation, and creating a podosome ring model for use with localization microscopy data.

KW - Image analysis

KW - Localization microscopy

KW - Pattern recognition

KW - Podosomes

KW - Super-resolution microscopy

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DO - 10.1016/j.ymeth.2016.11.005

M3 - Article

SN - 1046-2023

VL - 115

SP - 9

EP - 16

JO - Methods

JF - Methods

ER -

Investigation of podosome ring protein arrangement using localization microscopy images

Abstract

Keywords

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