King's College London

Research portal

Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats: UK and Galician Caucasian population surveys and usefulness in paternity investigations

Research output: Contribution to journalArticle

Standard

Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats : UK and Galician Caucasian population surveys and usefulness in paternity investigations. / Lareu, M V; Phillips, C P; Carracedo, A; Lincoln, P J; Syndercombe Court, D; Thomson, J. A.

In: Forensic Science International, Vol. 66, No. 1, 1994, p. 41-52.

Research output: Contribution to journalArticle

Harvard

Lareu, MV, Phillips, CP, Carracedo, A, Lincoln, PJ, Syndercombe Court, D & Thomson, JA 1994, 'Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats: UK and Galician Caucasian population surveys and usefulness in paternity investigations', Forensic Science International, vol. 66, no. 1, pp. 41-52. https://doi.org/10.1016/0379-0738(94)90318-2

APA

Lareu, M. V., Phillips, C. P., Carracedo, A., Lincoln, P. J., Syndercombe Court, D., & Thomson, J. A. (1994). Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats: UK and Galician Caucasian population surveys and usefulness in paternity investigations. Forensic Science International, 66(1), 41-52. https://doi.org/10.1016/0379-0738(94)90318-2

Vancouver

Lareu MV, Phillips CP, Carracedo A, Lincoln PJ, Syndercombe Court D, Thomson JA. Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats: UK and Galician Caucasian population surveys and usefulness in paternity investigations. Forensic Science International. 1994;66(1):41-52. https://doi.org/10.1016/0379-0738(94)90318-2

Author

Lareu, M V ; Phillips, C P ; Carracedo, A ; Lincoln, P J ; Syndercombe Court, D ; Thomson, J. A. / Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats : UK and Galician Caucasian population surveys and usefulness in paternity investigations. In: Forensic Science International. 1994 ; Vol. 66, No. 1. pp. 41-52.

Bibtex Download

@article{156d64cf7dd84339ad3dd1bb7e423a41,
title = "Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats: UK and Galician Caucasian population surveys and usefulness in paternity investigations",
abstract = "Two hundred unrelated Caucasians from the UK and 210 from Galicia (NW Spain) have been genotyped for the HUMTH01 locus using polymerase chain reaction (PCR) amplification followed by high sieving agarose electrophoresis (UK samples) and polyacrylamide electrophoresis (Galician samples). Allele and genotype frequencies obtained from both populations were in close agreement to those seen by other workers and chi 2 tests of the two populations showed that both were in Hardy-Weinberg equilibrium. The potential usefulness of HUMTH01 in paternity investigations was analysed by constructing, within each population, false family trios, each with a non-father, in order to estimate the actual exclusion rate. The observed rate of exclusion was in close agreement with the expected rate for both populations. Examination of the mother-child pairs in the false families showed a common allele in every case and no evidence of mutation or non-Mendelian inheritance was observed. The HUMTH01 locus shows an informative polymorphism and the production and analysis of population databases is easily achieved. The use of PCR followed by electrophoresis in either gel format appears to provide a quick and straightforward method for investigating the HUMTH01 locus.",
author = "Lareu, {M V} and Phillips, {C P} and A Carracedo and Lincoln, {P J} and {Syndercombe Court}, D and Thomson, {J. A.}",
year = "1994",
doi = "10.1016/0379-0738(94)90318-2",
language = "English",
volume = "66",
pages = "41--52",
journal = "Forensic Science International",
issn = "0379-0738",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Investigation of the STR locus HUMTH01 using PCR and two electrophoresis formats

T2 - UK and Galician Caucasian population surveys and usefulness in paternity investigations

AU - Lareu, M V

AU - Phillips, C P

AU - Carracedo, A

AU - Lincoln, P J

AU - Syndercombe Court, D

AU - Thomson, J. A.

PY - 1994

Y1 - 1994

N2 - Two hundred unrelated Caucasians from the UK and 210 from Galicia (NW Spain) have been genotyped for the HUMTH01 locus using polymerase chain reaction (PCR) amplification followed by high sieving agarose electrophoresis (UK samples) and polyacrylamide electrophoresis (Galician samples). Allele and genotype frequencies obtained from both populations were in close agreement to those seen by other workers and chi 2 tests of the two populations showed that both were in Hardy-Weinberg equilibrium. The potential usefulness of HUMTH01 in paternity investigations was analysed by constructing, within each population, false family trios, each with a non-father, in order to estimate the actual exclusion rate. The observed rate of exclusion was in close agreement with the expected rate for both populations. Examination of the mother-child pairs in the false families showed a common allele in every case and no evidence of mutation or non-Mendelian inheritance was observed. The HUMTH01 locus shows an informative polymorphism and the production and analysis of population databases is easily achieved. The use of PCR followed by electrophoresis in either gel format appears to provide a quick and straightforward method for investigating the HUMTH01 locus.

AB - Two hundred unrelated Caucasians from the UK and 210 from Galicia (NW Spain) have been genotyped for the HUMTH01 locus using polymerase chain reaction (PCR) amplification followed by high sieving agarose electrophoresis (UK samples) and polyacrylamide electrophoresis (Galician samples). Allele and genotype frequencies obtained from both populations were in close agreement to those seen by other workers and chi 2 tests of the two populations showed that both were in Hardy-Weinberg equilibrium. The potential usefulness of HUMTH01 in paternity investigations was analysed by constructing, within each population, false family trios, each with a non-father, in order to estimate the actual exclusion rate. The observed rate of exclusion was in close agreement with the expected rate for both populations. Examination of the mother-child pairs in the false families showed a common allele in every case and no evidence of mutation or non-Mendelian inheritance was observed. The HUMTH01 locus shows an informative polymorphism and the production and analysis of population databases is easily achieved. The use of PCR followed by electrophoresis in either gel format appears to provide a quick and straightforward method for investigating the HUMTH01 locus.

U2 - 10.1016/0379-0738(94)90318-2

DO - 10.1016/0379-0738(94)90318-2

M3 - Article

C2 - 7927087

VL - 66

SP - 41

EP - 52

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

IS - 1

ER -

View graph of relations

© 2018 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454