IRMS delta values (¹³C) of nandrolone and testosterone products available in the UK: Implications for anti-doping

TY - JOUR

T1 - IRMS delta values (13C) of nandrolone and testosterone products available in the UK

T2 - Implications for anti-doping

AU - Brailsford, Alan D.

AU - Majidin, Wan Noor Mahirah

AU - Wojek, Nick

AU - Cowan, David A.

AU - Walker, Christopher

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Anabolic androgenic steroids (AAS) are the most widely abused class of drugs by athletes and thus represent a significant problem to the anti-doping community. Confirmation of a doping violation for AAS cannot always be based on their presence alone due to the endogenous production of some steroids. Both testosterone (and its metabolites) and the major diagnostic metabolite of nandrolone (19-norandrosterone) are produced endogenously. Gas chromatography–combustion–isotope ratio mass spectrometry (GC–C–IRMS) is used in such cases to differentiate between the administration of a synthetic preparation and endogenous steroid production by measurement of their differing carbon isotope (13C/12C) ratio. The purpose of this study was to investigate the availability of steroid preparations in the UK with a 13C content analytically indistinguishable from that of endogenous steroids. Fourteen preparations containing nandrolone (n = 9) and testosterone (n = 5) were analyzed. The δ13C values were determined using GC–C–IRMS and the identity of the steroid preparations was confirmed using gas chromatography–mass spectrometry (GC–MS). Ten steroid preparations displayed δ13C values within the range expected for synthetic steroids (less than −27‰). However, four nandrolone preparations displayed δ13C values that overlap with the values considered to be endogenous in origin (range: −26 to −16‰). Misuse of these preparations could prevent the confirmation of nandrolone administration using GC–C–IRMS in anti-doping cases.

AB - Anabolic androgenic steroids (AAS) are the most widely abused class of drugs by athletes and thus represent a significant problem to the anti-doping community. Confirmation of a doping violation for AAS cannot always be based on their presence alone due to the endogenous production of some steroids. Both testosterone (and its metabolites) and the major diagnostic metabolite of nandrolone (19-norandrosterone) are produced endogenously. Gas chromatography–combustion–isotope ratio mass spectrometry (GC–C–IRMS) is used in such cases to differentiate between the administration of a synthetic preparation and endogenous steroid production by measurement of their differing carbon isotope (13C/12C) ratio. The purpose of this study was to investigate the availability of steroid preparations in the UK with a 13C content analytically indistinguishable from that of endogenous steroids. Fourteen preparations containing nandrolone (n = 9) and testosterone (n = 5) were analyzed. The δ13C values were determined using GC–C–IRMS and the identity of the steroid preparations was confirmed using gas chromatography–mass spectrometry (GC–MS). Ten steroid preparations displayed δ13C values within the range expected for synthetic steroids (less than −27‰). However, four nandrolone preparations displayed δ13C values that overlap with the values considered to be endogenous in origin (range: −26 to −16‰). Misuse of these preparations could prevent the confirmation of nandrolone administration using GC–C–IRMS in anti-doping cases.

KW - anti-doping

KW - GC–C–IRMS

KW - nandrolone

KW - seized preparations

KW - testosterone

UR - http://www.scopus.com/inward/record.url?scp=85057260476&partnerID=8YFLogxK

U2 - 10.1002/dta.2530

DO - 10.1002/dta.2530

M3 - Article

C2 - 30381908

AN - SCOPUS:85057260476

SN - 1942-7603

VL - 10

SP - 1722

EP - 1727

JO - Drug Testing And Analysis

JF - Drug Testing And Analysis

IS - 11-12

ER -