Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients

Athina Efthymiou, Nicoleta Mureanu, Rebecca Pemberton, Sarah Tai-macarthur, Daniela Mastronicola, Cristiano Scottà, Giovanna Lombardi, Kypros H. Nicolaides, Panicos Shangaris

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMateTM isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.
Original languageEnglish
Article number101204
JournalSTAR Protocols
Volume3
Issue number1
Early online date25 Feb 2022
DOIs
Publication statusPublished - 18 Mar 2022

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