Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)

TY - JOUR

T1 - Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)

AU - Warburton, Philip J

AU - Allan, Elaine

AU - Hunter, Stephanie

AU - Ward, John

AU - Booth, Veronica

AU - Wade, William G

AU - Mullany, Peter

N1 - © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

PY - 2011/11

Y1 - 2011/11

N2 - The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.

AB - The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.

U2 - 10.1111/j.1574-6941.2011.01166.x

DO - 10.1111/j.1574-6941.2011.01166.x

M3 - Article

C2 - 21711368

SN - 0168-6496

VL - 78

SP - 349

EP - 354

JO - FEMS MICROBIOLOGY ECOLOGY

JF - FEMS MICROBIOLOGY ECOLOGY

IS - 2

ER -