TY - JOUR
T1 - Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography
AU - Takov, Kaloyan
AU - Teng, I. Jou
AU - Mayr, Manuel
N1 - Funding Information:
M. M. is a British Heart Foundation (BHF) Chair Holder (CH/16/3/32406) with BHF programme grant support (RG/16/14/32397). This work was supported by ERA-CVD Transnational Grant ?MacroERA: Noncoding RNAs in cardiac macrophages and their role in heart failure,? and the Leducq Foundation (18CVD02).
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Circulating extracellular vesicles (EVs) are gaining increased attention as carriers of proteins, nucleic acids, and lipids. Blood contains EVs from different cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a single-step, high-performance size-exclusion chromatography procedure for separation of EVs from most lipoproteins and non-EV proteins, and compare it to ultracentrifugation, still the most commonly used method for EV isolation.
AB - Circulating extracellular vesicles (EVs) are gaining increased attention as carriers of proteins, nucleic acids, and lipids. Blood contains EVs from different cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a single-step, high-performance size-exclusion chromatography procedure for separation of EVs from most lipoproteins and non-EV proteins, and compare it to ultracentrifugation, still the most commonly used method for EV isolation.
KW - Exosomes
KW - Extracellular vesicles
KW - HPLC
KW - Lipoproteins
KW - Plasma
KW - Proteomics
KW - Size-exclusion chromatography
UR - http://www.scopus.com/inward/record.url?scp=85128801779&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2341-1_3
DO - 10.1007/978-1-0716-2341-1_3
M3 - Article
C2 - 35467277
AN - SCOPUS:85128801779
SN - 1940-6029
VL - 2504
SP - 31
EP - 40
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -