King's College London

Research portal

Isolation of single cells from mouse periodontal ligament

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Article numbere4120
Issue number16
Published20 Aug 2021

Bibliographical note

Funding Information: We thank Xinyu Jian for technical and administrative assistance during this work, in addition to all the members of the Macho laboratory for the constant improvement of this protocol and helpful discussions. Work in the Macho laboratory is supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB27040204), the Chinese 1000 Talents Program, and the Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences). Funding Information: We thank Dr. Lucia Zaugg, Dr. Arshiya Banu, and Dr. Jan Krivanek for their help. Jing Zhao holds training support from the China Scholarship Council Studentship with Kings College London. Funding from the NIHR GSTFT/KCL Biomedical Research Centre is acknowledged. The protocol was first described in our original research paper, ?Stem cell contributions to cementoblast differentiation in healthy periodontal ligament and periodontitis? published in Stem Cells (Zhao et al., 2021). Publisher Copyright: © 2021 The Authors; exclusive licensee Bio-protocol LLC. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

King's Authors


The periodontal ligament (PDL) is an essential tissue connecting teeth and bone. It is a complex tissue specifically designed to absorb the forces of mastication; analysis of its multiple cell populations is important to understand its function and the cell changes associated with periodontal disease. Cells in the periodontal ligament are not fully understood due to their physical location and small tissue size. It is challenging to isolate thin layers of cells compared with many other more substantial tissues. Here, we provide a straightforward protocol for the isolation of periodontal ligament cells from mice.

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454