Known and novel terpenes from Buddleja globosa displaying selective antifungal activity against dermatophytes

TY - JOUR

T1 - Known and novel terpenes from Buddleja globosa displaying selective antifungal activity against dermatophytes

AU - Mensah, A Y

AU - Houghton, P J

AU - Bloomfield, S

AU - Vlietinck, A

AU - Vanden Berghe, D

PY - 2000

Y1 - 2000

N2 - Lipophilic extracts of the stembark of Buddleja globosa were found to have antifungal activity at 125 mu g/mL against three dermatophytic fungal species but had no activity at 1000 mu g/mL against four other fungal species or two yeast species. Bioassay-guided fractionation of Si gel column eluates using the sensitive fungal species resulted in active fractions from which were isolated five compounds that were characterized by spectroscopic methods as one novel and four known compounds. The known compounds were the diterpene buddlejone (1), the bisditerpene maytenone, and the two sesquiterpenes buddledin A and buddledin B, while the novel compound was characterized as the diterpene deoxybuddlejone (2). The minimum inhibitory concentration of all the compounds was determined against all the microorganisms under test, and buddledins A and B were shown to exhibit the greatest antifungal activity, with values of 43 mu M and 51 mu M, respectively, against the sensitive fungi Trichophyton rubrum, Tricophyton interdigitale, and Epidermophyton floccosum.

AB - Lipophilic extracts of the stembark of Buddleja globosa were found to have antifungal activity at 125 mu g/mL against three dermatophytic fungal species but had no activity at 1000 mu g/mL against four other fungal species or two yeast species. Bioassay-guided fractionation of Si gel column eluates using the sensitive fungal species resulted in active fractions from which were isolated five compounds that were characterized by spectroscopic methods as one novel and four known compounds. The known compounds were the diterpene buddlejone (1), the bisditerpene maytenone, and the two sesquiterpenes buddledin A and buddledin B, while the novel compound was characterized as the diterpene deoxybuddlejone (2). The minimum inhibitory concentration of all the compounds was determined against all the microorganisms under test, and buddledins A and B were shown to exhibit the greatest antifungal activity, with values of 43 mu M and 51 mu M, respectively, against the sensitive fungi Trichophyton rubrum, Tricophyton interdigitale, and Epidermophyton floccosum.

UR - http://www.scopus.com/inward/record.url?scp=0033797955&partnerID=8YFLogxK

U2 - 10.1021/np0001023

DO - 10.1021/np0001023

M3 - Article

VL - 63

SP - 1210

EP - 1213

JO - Journal of Natural Products

JF - Journal of Natural Products

IS - 9

ER -