Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography

Mads Bergholt; Eileen Gentleman; Anders Walther; Dev Methrota; Magnus Jensen; Mu Yuan; Simon  Pedersen; Michael  Albro; Martin  Hedegaard

Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography

Mads Bergholt, Eileen Gentleman, Anders Walther, Dev Methrota, Magnus Jensen, Mu Yuan, Simon Pedersen, Michael Albro, Martin Hedegaard

Research output: Contribution to journal › Article › peer-review

Abstract

The ability to image tissues in three-dimensions (3-D) with label-free molecular contrast at mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3-D molecular contrast in transparent and semi-transparent samples. A computational pipeline for multivariate reconstruction was established to extract label-free spatial molecular information from Raman projection data. We demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a novel tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and live tissue-engineered constructs and explanted native tissues. RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.

Original language	English
Journal	Nature Communications
Publication status	Published - 2024

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title = "Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography",

abstract = "The ability to image tissues in three-dimensions (3-D) with label-free molecular contrast at mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3-D molecular contrast in transparent and semi-transparent samples. A computational pipeline for multivariate reconstruction was established to extract label-free spatial molecular information from Raman projection data. We demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a novel tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and live tissue-engineered constructs and explanted native tissues. RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.",

author = "Mads Bergholt and Eileen Gentleman and Anders Walther and Dev Methrota and Magnus Jensen and Mu Yuan and Simon Pedersen and Michael Albro and Martin Hedegaard",

year = "2024",

language = "English",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography

AU - Bergholt, Mads

AU - Gentleman, Eileen

AU - Walther, Anders

AU - Methrota, Dev

AU - Jensen, Magnus

AU - Yuan, Mu

AU - Pedersen, Simon

AU - Albro, Michael

AU - Hedegaard, Martin

PY - 2024

Y1 - 2024

N2 - The ability to image tissues in three-dimensions (3-D) with label-free molecular contrast at mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3-D molecular contrast in transparent and semi-transparent samples. A computational pipeline for multivariate reconstruction was established to extract label-free spatial molecular information from Raman projection data. We demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a novel tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and live tissue-engineered constructs and explanted native tissues. RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.

AB - The ability to image tissues in three-dimensions (3-D) with label-free molecular contrast at mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3-D molecular contrast in transparent and semi-transparent samples. A computational pipeline for multivariate reconstruction was established to extract label-free spatial molecular information from Raman projection data. We demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a novel tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and live tissue-engineered constructs and explanted native tissues. RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.

M3 - Article

SN - 2041-1723

JO - Nature Communications

JF - Nature Communications

ER -

Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography

Abstract

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