We report onwide-field time-correlated single photon counting (TCSPC)-based fluorescence
lifetime imagingmicroscopy (FLIM) with lightsheet illumination.A pulsed
diode laser is used for excitation, and a crossed delay line anode image intensifier,
effectively a single-photon sensitive camera, is used to record the position and arrival
time of the photons with picosecond time resolution, combining low illumination
intensity of microwattswith wide-field data collection.We pair this detector with the
lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients
in human cancer cell spheroids, and C. elegans.
Original languageEnglish
Article numbere201960099
JournalJournal of biophotonics
Publication statusAccepted/In press - 2019


  • SPIM
  • fluorescence lifetime imaging (FLIM)
  • lightsheet microscopy
  • microchannel plate (MCP)
  • time-correlated single photon counting (TCSPC)


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