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Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila

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Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila. / Bridi, Jessika Cristina; Ludlow, Zoe; Hirth, Frank.

In: Biology Open, Vol. 8, No. 7, 045062, 08.07.2019.

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Harvard

Bridi, JC, Ludlow, Z & Hirth, F 2019, 'Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila', Biology Open, vol. 8, no. 7, 045062. https://doi.org/10.1242/bio.045062

APA

Bridi, J. C., Ludlow, Z., & Hirth, F. (2019). Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila. Biology Open, 8(7), [045062]. https://doi.org/10.1242/bio.045062

Vancouver

Bridi JC, Ludlow Z, Hirth F. Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila. Biology Open. 2019 Jul 8;8(7). 045062. https://doi.org/10.1242/bio.045062

Author

Bridi, Jessika Cristina ; Ludlow, Zoe ; Hirth, Frank. / Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila. In: Biology Open. 2019 ; Vol. 8, No. 7.

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@article{f7471ec97ea94bc2bf09c53f5e9175e9,
title = "Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila",
abstract = "The ellipsoid body (EB) of the Drosophila central complex mediates sensorimotor 33 integration and action selection for adaptive behaviours. Insights into its physiological 34 function are steadily accumulating, however the developmental origin and genetic 35 specification have remained largely elusive. Here we identify two stem cells in the 36 embryonic neuroectoderm as precursor cells of neuronal progeny that establish EB 37 circuits in the adult brain. Genetic tracing of embryonic neuroblasts ppd5 and mosaic 38 analysis with a repressible cell marker identified lineage-related progeny as Pox neuro 39 (Poxn)-expressing EB ring neurons, R1-R4. During embryonic brain development, 40 engrailed function is required for the initial formation of Poxn-expressing ppd5-derived 41 progeny. Postembryonic determination of R1-R4 identity depends on lineage-specific 42 Poxn function that separates neuronal subtypes of ppd5-derived progeny into hemi-43 lineages with projections either terminating in the EB ring neuropil or the superior 44 protocerebrum (SP). Poxn knockdown in ppd5-derived progeny results in identity 45 transformation of engrailed-expressing hemi-lineages from SP to EB-specific circuits. In 46 contrast, lineage-specific knockdown of engrailed leads to reduced numbers of Poxn-47 expressing ring neurons. These findings establish neuroblasts ppd5-derived ring 48 neurons as lineage-related sister cells that require engrailed and Poxn function for the 49 proper formation of EB circuitry in the adult central complex of Drosophila.",
keywords = "Brain, Central complex, Drosophila, Ellipsoid body, Neural circuit, Stem cell lineage",
author = "Bridi, {Jessika Cristina} and Zoe Ludlow and Frank Hirth",
year = "2019",
month = jul,
day = "8",
doi = "10.1242/bio.045062",
language = "English",
volume = "8",
journal = "Biology Open",
issn = "2046-6390",
publisher = "Company of Biologists Ltd",
number = "7",

}

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TY - JOUR

T1 - Lineage-specific determination of ring neuron circuitry in the central complex of Drosophila

AU - Bridi, Jessika Cristina

AU - Ludlow, Zoe

AU - Hirth, Frank

PY - 2019/7/8

Y1 - 2019/7/8

N2 - The ellipsoid body (EB) of the Drosophila central complex mediates sensorimotor 33 integration and action selection for adaptive behaviours. Insights into its physiological 34 function are steadily accumulating, however the developmental origin and genetic 35 specification have remained largely elusive. Here we identify two stem cells in the 36 embryonic neuroectoderm as precursor cells of neuronal progeny that establish EB 37 circuits in the adult brain. Genetic tracing of embryonic neuroblasts ppd5 and mosaic 38 analysis with a repressible cell marker identified lineage-related progeny as Pox neuro 39 (Poxn)-expressing EB ring neurons, R1-R4. During embryonic brain development, 40 engrailed function is required for the initial formation of Poxn-expressing ppd5-derived 41 progeny. Postembryonic determination of R1-R4 identity depends on lineage-specific 42 Poxn function that separates neuronal subtypes of ppd5-derived progeny into hemi-43 lineages with projections either terminating in the EB ring neuropil or the superior 44 protocerebrum (SP). Poxn knockdown in ppd5-derived progeny results in identity 45 transformation of engrailed-expressing hemi-lineages from SP to EB-specific circuits. In 46 contrast, lineage-specific knockdown of engrailed leads to reduced numbers of Poxn-47 expressing ring neurons. These findings establish neuroblasts ppd5-derived ring 48 neurons as lineage-related sister cells that require engrailed and Poxn function for the 49 proper formation of EB circuitry in the adult central complex of Drosophila.

AB - The ellipsoid body (EB) of the Drosophila central complex mediates sensorimotor 33 integration and action selection for adaptive behaviours. Insights into its physiological 34 function are steadily accumulating, however the developmental origin and genetic 35 specification have remained largely elusive. Here we identify two stem cells in the 36 embryonic neuroectoderm as precursor cells of neuronal progeny that establish EB 37 circuits in the adult brain. Genetic tracing of embryonic neuroblasts ppd5 and mosaic 38 analysis with a repressible cell marker identified lineage-related progeny as Pox neuro 39 (Poxn)-expressing EB ring neurons, R1-R4. During embryonic brain development, 40 engrailed function is required for the initial formation of Poxn-expressing ppd5-derived 41 progeny. Postembryonic determination of R1-R4 identity depends on lineage-specific 42 Poxn function that separates neuronal subtypes of ppd5-derived progeny into hemi-43 lineages with projections either terminating in the EB ring neuropil or the superior 44 protocerebrum (SP). Poxn knockdown in ppd5-derived progeny results in identity 45 transformation of engrailed-expressing hemi-lineages from SP to EB-specific circuits. In 46 contrast, lineage-specific knockdown of engrailed leads to reduced numbers of Poxn-47 expressing ring neurons. These findings establish neuroblasts ppd5-derived ring 48 neurons as lineage-related sister cells that require engrailed and Poxn function for the 49 proper formation of EB circuitry in the adult central complex of Drosophila.

KW - Brain

KW - Central complex

KW - Drosophila

KW - Ellipsoid body

KW - Neural circuit

KW - Stem cell lineage

UR - http://www.scopus.com/inward/record.url?scp=85070565589&partnerID=8YFLogxK

U2 - 10.1242/bio.045062

DO - 10.1242/bio.045062

M3 - Article

VL - 8

JO - Biology Open

JF - Biology Open

SN - 2046-6390

IS - 7

M1 - 045062

ER -

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