TY - JOUR
T1 - Lipids in biocalcification: contrasts and similarities between intimal and medial vascular calcification and bone by NMR
AU - Reid, David G.
AU - Shanahan, Catherine M.
AU - Duer, Melinda J.
AU - Arroyo, Luis G.
AU - Schoppet, Michael
AU - Brooks, Roger A.
AU - Murray, Rachel C.
PY - 2012/8
Y1 - 2012/8
N2 - Pathomechanisms underlying vascular calcification biogenesis are still incompletely understood. Biomineral from human atherosclerotic intimal plaques; human, equine, and bovine medial vascular calcifications; and human and equine bone was released from collagenous organic matrix by sodium hydroxide/sodium hypochlorite digestion. Solid-state C-13 NMR of intimal plaque mineral shows signals from cholesterol/cholesteryl esters and fatty acids. In contrast, in mineral from pure medial calcifications and bone mineral, fatty acid signals predominate. Refluxing (chloroform/methanol) intimal plaque calcifications removes the cholesterylic but not the fatty acyl signals. The lipid composition of this refluxed mineral now closely resembles that of the medial and bone mineral, which is unchanged by reflux. Thus, intimal and medial vascular calcifications and bone mineral have in common a pool of occluded mineral-entrained fatty acyl-rich lipids. This population of fatty acid may contain methyl-branched fatty acids, possibly representing lipoprotein particle remnants. Cell signaling and mechanistic parallels between physiological (orthotopic) and pathological (ectopic) calcification are also reflected thus in the NMR spectroscopic fingerprints of mineral-associated and mineral-entrained lipids. Additionally the atherosclerotic plaque mineral alone shows a significant independent pool of cholesterylic lipids.(jlr) Colocalization of mineral and lipid may be coincidental, but it could also reflect an essential mechanistic component of biomineralization.-Reid, D. G., C. M. Shanahan, M. J. Duer, L. G. Arroyo, M. Schoppet, R. A. Brooks, and R. C. Murray. Lipids in biocalcification: contrasts and similarities between intimal and medial vascular calcification and bone by NMR. J. Lipid Res. 2012. 53: 1569-1575.
AB - Pathomechanisms underlying vascular calcification biogenesis are still incompletely understood. Biomineral from human atherosclerotic intimal plaques; human, equine, and bovine medial vascular calcifications; and human and equine bone was released from collagenous organic matrix by sodium hydroxide/sodium hypochlorite digestion. Solid-state C-13 NMR of intimal plaque mineral shows signals from cholesterol/cholesteryl esters and fatty acids. In contrast, in mineral from pure medial calcifications and bone mineral, fatty acid signals predominate. Refluxing (chloroform/methanol) intimal plaque calcifications removes the cholesterylic but not the fatty acyl signals. The lipid composition of this refluxed mineral now closely resembles that of the medial and bone mineral, which is unchanged by reflux. Thus, intimal and medial vascular calcifications and bone mineral have in common a pool of occluded mineral-entrained fatty acyl-rich lipids. This population of fatty acid may contain methyl-branched fatty acids, possibly representing lipoprotein particle remnants. Cell signaling and mechanistic parallels between physiological (orthotopic) and pathological (ectopic) calcification are also reflected thus in the NMR spectroscopic fingerprints of mineral-associated and mineral-entrained lipids. Additionally the atherosclerotic plaque mineral alone shows a significant independent pool of cholesterylic lipids.(jlr) Colocalization of mineral and lipid may be coincidental, but it could also reflect an essential mechanistic component of biomineralization.-Reid, D. G., C. M. Shanahan, M. J. Duer, L. G. Arroyo, M. Schoppet, R. A. Brooks, and R. C. Murray. Lipids in biocalcification: contrasts and similarities between intimal and medial vascular calcification and bone by NMR. J. Lipid Res. 2012. 53: 1569-1575.
U2 - 10.1194/jlr.M026088
DO - 10.1194/jlr.M026088
M3 - Article
SN - 0022-2275
VL - 53
SP - 1569
EP - 1575
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 8
ER -
