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Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: Role of RFamide-related peptide-3 and kisspeptin

Research output: Contribution to journalArticle

Chooi Yeng Lee, ShengYun Li, Xiao Feng Li, Daniel A.E. Stalker, Claire Cooke, Bei Shao, Haluk Kelestimur, Belinda A. Henry, Gregory Conductier, Kevin T. O'Byrne, Iain J. Clarke

Original languageEnglish
Pages (from-to)1134-1143
Number of pages10
JournalReproduction, Fertility and Development
Issue number6
Publication statusPublished - 1 Jan 2019

King's Authors


RFamide-related peptide (RFRP)-3 reduces luteinising hormone (LH) secretion in rodents. Stress has been shown to upregulate the expression of the RFRP gene (Rfrp) with a concomitant reduction in LH secretion, but an effect on expression of the gonadotrophin-releasing hormone (GnRH) gene (Gnrh1) has not been shown. We hypothesised that lipopolysaccharide (LPS)-induced stress affects expression of Rfrp, the gene for kisspeptin (Kiss1) and/or Gnrh1, leading to suppression of LH levels in rats. Intracerebroventricular injections of RFRP-3 (0.1, 1, 5 nmol) or i.v. LPS (15 μg kg -1 ) reduced LH levels. Doses of 1 and 5 nmol RFRP-3 were then administered to analyse gene expression by in situ hybridisation. RFRP-3 (5 nmol) had no effect on Gnrh1 or Kiss1 expression. LPS stress reduced GnRH and Kiss1 expression, without affecting Rfrp1 expression. These data indicate that LPS stress directly or indirectly reduces Gnrh1 expression, but this is unlikely to be due to a change in Rfrp1 expression.

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