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Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: Role of RFamide-related peptide-3 and kisspeptin

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Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression : Role of RFamide-related peptide-3 and kisspeptin. / Lee, Chooi Yeng; Li, ShengYun; Li, Xiao Feng; Stalker, Daniel A.E.; Cooke, Claire; Shao, Bei; Kelestimur, Haluk; Henry, Belinda A.; Conductier, Gregory; O'Byrne, Kevin T.; Clarke, Iain J.

In: Reproduction, Fertility and Development, Vol. 31, No. 6, 01.01.2019, p. 1134-1143.

Research output: Contribution to journalArticle

Harvard

Lee, CY, Li, S, Li, XF, Stalker, DAE, Cooke, C, Shao, B, Kelestimur, H, Henry, BA, Conductier, G, O'Byrne, KT & Clarke, IJ 2019, 'Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: Role of RFamide-related peptide-3 and kisspeptin', Reproduction, Fertility and Development, vol. 31, no. 6, pp. 1134-1143. https://doi.org/10.1071/RD18277

APA

Lee, C. Y., Li, S., Li, X. F., Stalker, D. A. E., Cooke, C., Shao, B., Kelestimur, H., Henry, B. A., Conductier, G., O'Byrne, K. T., & Clarke, I. J. (2019). Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: Role of RFamide-related peptide-3 and kisspeptin. Reproduction, Fertility and Development, 31(6), 1134-1143. https://doi.org/10.1071/RD18277

Vancouver

Lee CY, Li S, Li XF, Stalker DAE, Cooke C, Shao B et al. Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: Role of RFamide-related peptide-3 and kisspeptin. Reproduction, Fertility and Development. 2019 Jan 1;31(6):1134-1143. https://doi.org/10.1071/RD18277

Author

Lee, Chooi Yeng ; Li, ShengYun ; Li, Xiao Feng ; Stalker, Daniel A.E. ; Cooke, Claire ; Shao, Bei ; Kelestimur, Haluk ; Henry, Belinda A. ; Conductier, Gregory ; O'Byrne, Kevin T. ; Clarke, Iain J. / Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression : Role of RFamide-related peptide-3 and kisspeptin. In: Reproduction, Fertility and Development. 2019 ; Vol. 31, No. 6. pp. 1134-1143.

Bibtex Download

@article{96babe4a72db4278bbca02bb42287030,
title = "Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: Role of RFamide-related peptide-3 and kisspeptin",
abstract = " RFamide-related peptide (RFRP)-3 reduces luteinising hormone (LH) secretion in rodents. Stress has been shown to upregulate the expression of the RFRP gene (Rfrp) with a concomitant reduction in LH secretion, but an effect on expression of the gonadotrophin-releasing hormone (GnRH) gene (Gnrh1) has not been shown. We hypothesised that lipopolysaccharide (LPS)-induced stress affects expression of Rfrp, the gene for kisspeptin (Kiss1) and/or Gnrh1, leading to suppression of LH levels in rats. Intracerebroventricular injections of RFRP-3 (0.1, 1, 5 nmol) or i.v. LPS (15 μg kg -1 ) reduced LH levels. Doses of 1 and 5 nmol RFRP-3 were then administered to analyse gene expression by in situ hybridisation. RFRP-3 (5 nmol) had no effect on Gnrh1 or Kiss1 expression. LPS stress reduced GnRH and Kiss1 expression, without affecting Rfrp1 expression. These data indicate that LPS stress directly or indirectly reduces Gnrh1 expression, but this is unlikely to be due to a change in Rfrp1 expression. ",
keywords = "hypothalamus, neuroendocrinology, neuropeptides, stress",
author = "Lee, {Chooi Yeng} and ShengYun Li and Li, {Xiao Feng} and Stalker, {Daniel A.E.} and Claire Cooke and Bei Shao and Haluk Kelestimur and Henry, {Belinda A.} and Gregory Conductier and O'Byrne, {Kevin T.} and Clarke, {Iain J.}",
year = "2019",
month = jan,
day = "1",
doi = "10.1071/RD18277",
language = "English",
volume = "31",
pages = "1134--1143",
journal = "Reproduction Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "6",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression

T2 - Role of RFamide-related peptide-3 and kisspeptin

AU - Lee, Chooi Yeng

AU - Li, ShengYun

AU - Li, Xiao Feng

AU - Stalker, Daniel A.E.

AU - Cooke, Claire

AU - Shao, Bei

AU - Kelestimur, Haluk

AU - Henry, Belinda A.

AU - Conductier, Gregory

AU - O'Byrne, Kevin T.

AU - Clarke, Iain J.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - RFamide-related peptide (RFRP)-3 reduces luteinising hormone (LH) secretion in rodents. Stress has been shown to upregulate the expression of the RFRP gene (Rfrp) with a concomitant reduction in LH secretion, but an effect on expression of the gonadotrophin-releasing hormone (GnRH) gene (Gnrh1) has not been shown. We hypothesised that lipopolysaccharide (LPS)-induced stress affects expression of Rfrp, the gene for kisspeptin (Kiss1) and/or Gnrh1, leading to suppression of LH levels in rats. Intracerebroventricular injections of RFRP-3 (0.1, 1, 5 nmol) or i.v. LPS (15 μg kg -1 ) reduced LH levels. Doses of 1 and 5 nmol RFRP-3 were then administered to analyse gene expression by in situ hybridisation. RFRP-3 (5 nmol) had no effect on Gnrh1 or Kiss1 expression. LPS stress reduced GnRH and Kiss1 expression, without affecting Rfrp1 expression. These data indicate that LPS stress directly or indirectly reduces Gnrh1 expression, but this is unlikely to be due to a change in Rfrp1 expression.

AB - RFamide-related peptide (RFRP)-3 reduces luteinising hormone (LH) secretion in rodents. Stress has been shown to upregulate the expression of the RFRP gene (Rfrp) with a concomitant reduction in LH secretion, but an effect on expression of the gonadotrophin-releasing hormone (GnRH) gene (Gnrh1) has not been shown. We hypothesised that lipopolysaccharide (LPS)-induced stress affects expression of Rfrp, the gene for kisspeptin (Kiss1) and/or Gnrh1, leading to suppression of LH levels in rats. Intracerebroventricular injections of RFRP-3 (0.1, 1, 5 nmol) or i.v. LPS (15 μg kg -1 ) reduced LH levels. Doses of 1 and 5 nmol RFRP-3 were then administered to analyse gene expression by in situ hybridisation. RFRP-3 (5 nmol) had no effect on Gnrh1 or Kiss1 expression. LPS stress reduced GnRH and Kiss1 expression, without affecting Rfrp1 expression. These data indicate that LPS stress directly or indirectly reduces Gnrh1 expression, but this is unlikely to be due to a change in Rfrp1 expression.

KW - hypothalamus

KW - neuroendocrinology

KW - neuropeptides

KW - stress

UR - http://www.scopus.com/inward/record.url?scp=85063891303&partnerID=8YFLogxK

U2 - 10.1071/RD18277

DO - 10.1071/RD18277

M3 - Article

C2 - 30922440

AN - SCOPUS:85063891303

VL - 31

SP - 1134

EP - 1143

JO - Reproduction Fertility and Development

JF - Reproduction Fertility and Development

SN - 1031-3613

IS - 6

ER -

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