King's College London

Research portal

Live cell ATR-FTIR spectroscopy as a novel bioanalytical tool for cell glucose metabolism research

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Article number119024
Pages (from-to)119024
JournalBiochimica et biophysica acta-Molecular cell research
Volume1868
Issue number7
Early online date5 Apr 2021
DOIs
Accepted/In press26 Mar 2021
E-pub ahead of print5 Apr 2021
PublishedJun 2021

Bibliographical note

Funding Information: We thank Prof Khuloud Al-Jamal's research group for helps and advice on cell culturing. We thank Dr Andrei Tarasov from Oxford for the stimulating discussion. We thank Dr Ali Altharawi for general support. We also thank the Thai Government for providing a PhD studentship for Anchisa Poonprasartporn to carry out this research. We also thank EPSRC (EP/L013045/1) for the funding that started this research theme in the group. Publisher Copyright: © 2021 Elsevier B.V. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

Documents

King's Authors

Abstract

Current novel drug developments for the treatment of diabetes require multiple bioanalytical assays to interrogate the cellular metabolism, which are costly, laborious and time-consuming. Fourier-transform infrared (FTIR) spectroscopy is a nondestructive, label-free, sensitive and low-cost technique that is recently found to be suitable for studying living cells. The aim of this study is to demonstrate that live-cell FTIR can be applied to study the differences in glucose metabolism in cells in normal culturing medium and cells treated in high glucose (a diabetes model) in order to highlight the potential of the technique in diabetes research. Live HepG2 cells were treated in normal glucose (3.8 mM; control) or high glucose (25 mM) medium and were measured directly using the FTIR approach. Principal component analysis was used to highlight any possible correlated changes 24, 48 and 72 h after treatments. FTIR spectra of live cell treated in normal and high glucose medium have shown significant differences (p < 0.05) for all treatment time. The control cells have seen an increased in the absorbance at 1088, 1240 and 1400 cm −1, which are associated with phosphate stretching mode vibrations from phosphorylated proteins and DNA back bone; and symmetric stretching mode vibration of COO - from fatty acids, amino acids, lipids and carbohydrate metabolites. However, the high glucose treated cells have shown a different changes in the 1000–1200 cm −1 region, which is linked to the glycogen and ATP:ADP ratio. In conclusion, live-cell FTIR can be a low-cost method for the studies of metabolic changes in cells.

Download statistics

No data available

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454