Live cell imaging analysis of receptor function

TY - JOUR

T1 - Live cell imaging analysis of receptor function

AU - Worth, Daniel. C

AU - Parsons, Maddy

PY - 2010

Y1 - 2010

N2 - Cell surface receptors are crucial in the regulation of a wide variety of signalling responses to extracellular stimuli such as soluble growth factors or matrix proteins. To respond effectively to rapidly changing environmental cues, many receptors are rapidly endo- or exo-cytosed to either subcellular or membrane compartments or they recruit specific intracellular binding partners. Recent advances in microscopy techniques have made it possible to study receptor behaviour in live cells to gain a better understanding of dynamics, binding partners and sub-cellular localisation. Here we describe several common currently used techniques to study receptor behaviour in living cells.

AB - Cell surface receptors are crucial in the regulation of a wide variety of signalling responses to extracellular stimuli such as soluble growth factors or matrix proteins. To respond effectively to rapidly changing environmental cues, many receptors are rapidly endo- or exo-cytosed to either subcellular or membrane compartments or they recruit specific intracellular binding partners. Recent advances in microscopy techniques have made it possible to study receptor behaviour in live cells to gain a better understanding of dynamics, binding partners and sub-cellular localisation. Here we describe several common currently used techniques to study receptor behaviour in living cells.

U2 - 10.1007/978-1-60761-404-3_18

DO - 10.1007/978-1-60761-404-3_18

M3 - Article

VL - 591

SP - 311

EP - 323

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

IS - 2

ER -