Local origin of cells in FGF-4 - induced outgrowth of amputated chick wing bud stumps

K Kostakopoulou; N Vargesson; J D Clarke; P M Brickell; C Tickle

Local origin of cells in FGF-4 - induced outgrowth of amputated chick wing bud stumps

K Kostakopoulou, N Vargesson, J D Clarke, P M Brickell, C Tickle

Developmental Neurobiology

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Urodele amphibians are the only vertebrates that can regenerate amputated limbs, even as adults. However, we have previously shown that amputated chick wing bud stumps can be induced to ((regenerate)) and to form a complete set of correctly-patterned skeletal elements, following implantation of beads soaked in fibroblast growth factor-4 (FGF-4). We have now performed Dil injection experiments to determine which cells contribute to FGF-4-induced chick wing bud ((regenerates)). We show that the FGF-4-induced outgrowth of the regenerating wing bud stump is comprised of mesenchyme cells that originate from a region within 200 microm of the FGF-4 bead, and that cells proximal to the bead move distally.

Original language	English
Pages (from-to)	747-50
Number of pages	4
Journal	International Journal of Developmental Biology
Volume	41
Issue number	5
Publication status	Published - Oct 1997

Keywords

Animals
Proto-Oncogene Proteins
Limb Buds
Chick Embryo
Regeneration
Microinjections
Fluorescent Dyes
Wing
Mesoderm
Fibroblast Growth Factors
Fibroblast Growth Factor 4
Carbocyanines

Cite this

@article{7029b88e08ba4123b74df3c845ed7db4,

title = "Local origin of cells in FGF-4 - induced outgrowth of amputated chick wing bud stumps",

abstract = "Urodele amphibians are the only vertebrates that can regenerate amputated limbs, even as adults. However, we have previously shown that amputated chick wing bud stumps can be induced to ((regenerate)) and to form a complete set of correctly-patterned skeletal elements, following implantation of beads soaked in fibroblast growth factor-4 (FGF-4). We have now performed Dil injection experiments to determine which cells contribute to FGF-4-induced chick wing bud ((regenerates)). We show that the FGF-4-induced outgrowth of the regenerating wing bud stump is comprised of mesenchyme cells that originate from a region within 200 microm of the FGF-4 bead, and that cells proximal to the bead move distally.",

keywords = "Animals, Proto-Oncogene Proteins, Limb Buds, Chick Embryo, Regeneration, Microinjections, Fluorescent Dyes, Wing, Mesoderm, Fibroblast Growth Factors, Fibroblast Growth Factor 4, Carbocyanines",

author = "K Kostakopoulou and N Vargesson and Clarke, {J D} and Brickell, {P M} and C Tickle",

year = "1997",

month = oct,

language = "English",

volume = "41",

pages = "747--50",

journal = "International Journal of Developmental Biology",

issn = "0214-6282",

publisher = "University of the Basque Country Press",

number = "5",

}

TY - JOUR

T1 - Local origin of cells in FGF-4 - induced outgrowth of amputated chick wing bud stumps

AU - Kostakopoulou, K

AU - Vargesson, N

AU - Clarke, J D

AU - Brickell, P M

AU - Tickle, C

PY - 1997/10

Y1 - 1997/10

N2 - Urodele amphibians are the only vertebrates that can regenerate amputated limbs, even as adults. However, we have previously shown that amputated chick wing bud stumps can be induced to ((regenerate)) and to form a complete set of correctly-patterned skeletal elements, following implantation of beads soaked in fibroblast growth factor-4 (FGF-4). We have now performed Dil injection experiments to determine which cells contribute to FGF-4-induced chick wing bud ((regenerates)). We show that the FGF-4-induced outgrowth of the regenerating wing bud stump is comprised of mesenchyme cells that originate from a region within 200 microm of the FGF-4 bead, and that cells proximal to the bead move distally.

AB - Urodele amphibians are the only vertebrates that can regenerate amputated limbs, even as adults. However, we have previously shown that amputated chick wing bud stumps can be induced to ((regenerate)) and to form a complete set of correctly-patterned skeletal elements, following implantation of beads soaked in fibroblast growth factor-4 (FGF-4). We have now performed Dil injection experiments to determine which cells contribute to FGF-4-induced chick wing bud ((regenerates)). We show that the FGF-4-induced outgrowth of the regenerating wing bud stump is comprised of mesenchyme cells that originate from a region within 200 microm of the FGF-4 bead, and that cells proximal to the bead move distally.

KW - Animals

KW - Proto-Oncogene Proteins

KW - Limb Buds

KW - Chick Embryo

KW - Regeneration

KW - Microinjections

KW - Fluorescent Dyes

KW - Wing

KW - Mesoderm

KW - Fibroblast Growth Factors

KW - Fibroblast Growth Factor 4

KW - Carbocyanines

M3 - Article

C2 - 9415496

SN - 0214-6282

VL - 41

SP - 747

EP - 750

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

IS - 5

ER -

Local origin of cells in FGF-4 - induced outgrowth of amputated chick wing bud stumps

Abstract

Keywords

Fingerprint

Cite this