Low power super resolution fluorescence microscopy by lifetime modification and image reconstruction

TY - JOUR

T1 - Low power super resolution fluorescence microscopy by lifetime modification and image reconstruction

AU - Marsh, Richard J.

AU - Culley, Siân

AU - Bain, Angus J.

PY - 2014

Y1 - 2014

N2 - We demonstrate a new method for obtaining sub-diffraction resolution in fluorescence microscopy. The technique involves the analysis of the time evolution of fluorescence images in the presence of weak and unstructured (fundamental Gaussian) continuous wave stimulated emission depletion. A reduced point spread functions (PSF) is obtained by the recombination of time segments of the evolving image. A significant reduction in the PSF for 20nm fluorescent beads (ca. 240nm to 125nm) is obtained with an on-sample power of 7.5mW (17MW/cm2) - substantially lower than that required for spatially structured stimulated emission depletion microscopy.

AB - We demonstrate a new method for obtaining sub-diffraction resolution in fluorescence microscopy. The technique involves the analysis of the time evolution of fluorescence images in the presence of weak and unstructured (fundamental Gaussian) continuous wave stimulated emission depletion. A reduced point spread functions (PSF) is obtained by the recombination of time segments of the evolving image. A significant reduction in the PSF for 20nm fluorescent beads (ca. 240nm to 125nm) is obtained with an on-sample power of 7.5mW (17MW/cm2) - substantially lower than that required for spatially structured stimulated emission depletion microscopy.

UR - http://www.scopus.com/inward/record.url?scp=84901283013&partnerID=8YFLogxK

U2 - 10.1364/OE.22.012327

DO - 10.1364/OE.22.012327

M3 - Article

C2 - 24921351

AN - SCOPUS:84901283013

SN - 1094-4087

VL - 22

SP - 12327

EP - 12338

JO - OPTICS EXPRESS

JF - OPTICS EXPRESS

IS - 10

ER -