Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation

Katharina Isabelle Kober; Amparo Cano; Cyrill Géraud; Kalle Sipilä; Seyedeh Atefeh Mobasseri; Christina Philippeos; Angela Oliveira Pisco; Andrew Stannard; Alberto Martin; Fernando Salvador; Vanesa Santos; Michael Boutros; Emanuel Rognoni; Fiona M Watt

doi:10.1371/journal.pone.0199679

Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation

Katharina Isabelle Kober, Amparo Cano, Cyrill Géraud, Kalle Sipilä, Seyedeh Atefeh Mobasseri, Christina Philippeos, Angela Oliveira Pisco, Andrew Stannard, Alberto Martin, Fernando Salvador, Vanesa Santos, Michael Boutros, Emanuel Rognoni^*, Fiona M Watt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phorbol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.

Original language	English
Article number	e0199679
Journal	PLoS ONE
Volume	13
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0199679
Publication status	Published - 28 Jun 2018

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@article{7ce867758566458aaff283de0dfbc597,

title = "Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation",

abstract = "Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phorbol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.",

author = "Kober, {Katharina Isabelle} and Amparo Cano and Cyrill G{\'e}raud and Kalle Sipil{\"a} and Mobasseri, {Seyedeh Atefeh} and Christina Philippeos and Pisco, {Angela Oliveira} and Andrew Stannard and Alberto Martin and Fernando Salvador and Vanesa Santos and Michael Boutros and Emanuel Rognoni and Watt, {Fiona M}",

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doi = "10.1371/journal.pone.0199679",

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T1 - Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation

AU - Kober, Katharina Isabelle

AU - Cano, Amparo

AU - Géraud, Cyrill

AU - Sipilä, Kalle

AU - Mobasseri, Seyedeh Atefeh

AU - Philippeos, Christina

AU - Pisco, Angela Oliveira

AU - Stannard, Andrew

AU - Martin, Alberto

AU - Salvador, Fernando

AU - Santos, Vanesa

AU - Boutros, Michael

AU - Rognoni, Emanuel

AU - Watt, Fiona M

PY - 2018/6/28

Y1 - 2018/6/28

N2 - Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phorbol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.

AB - Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phorbol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.

UR - http://www.scopus.com/inward/record.url?scp=85049150702&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0199679

DO - 10.1371/journal.pone.0199679

M3 - Article

C2 - 29953488

SN - 1932-6203

VL - 13

JO - PLoS ONE

JF - PLoS ONE

IS - 6

M1 - e0199679

ER -

Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation

Abstract

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