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Lung heterogeneity and deadspace volume in acute respiratory distress syndrome animals using the inspired sinewave test

Research output: Contribution to journalArticle

Minh Tran, Douglas C Crockett, Federico Formenti, Phi Phan, Goran Hedenstierna, Anders Larsson, Stephen Payne, Andrew Farmery

Original languageEnglish
JournalPhysiological Measurement
Accepted/In press13 Oct 2020


King's Authors


The acute respiratory distress syndrome is associated with a high rate of morbidity and mortality, as patients undergoing mechanical ventilation are at risk of ventilator-induced lung injuries. Objective: To measure the lung heterogeneity and deadspace volume to and safer ventilator strategies. Then, the ventilator settings could over homogeneous ventilation and theoretically equalise and reduce tidal strain/stress in the lung parenchyma. Approach: The Inspired Sinewave Test (IST) is a non-invasive lung measurement tool, which does not require patients' cooperation. The IST can measure the effective lung volume, pulmonary blood flow and deadspace volume. We developed a computational simulation of the cardiopulmonary system to allow lung heterogeneity to be quantified using data solely derived from the IST. Then, the method to quantify lung heterogeneity using two IST tracer gas frequencies (180s and 60s) was introduced and experimented in the simulation lungs and in animal models. Thirteen anaesthetised pigs were studied with the IST, both before and after experimental lung injury (saline-lavage ARDS model). The deadspace volume is compared between IST and the SF6 Washout method. Results: The IST could measure the lung heterogeneity using two frequencies tracer gases. Furthermore, the value of IST ventilation heterogeneity in ARDS lungs were higher than in control lungs at PEEP 10cmH2O (AuC = 0.85, p < 0.001). Deadspace volume values measured by the IST has a strong relationship with the measured values of the SF6 (9mL bias and limits of agreement from −79mL to 57mL in control animals). Significance: the potential impact of the IST technique in the identification of ventilation and perfusion heterogeneity during ventilator support.

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