Magnetic sentinel lymph node biopsy in a murine tumour model

Muneer Ahmed*, Taeseong Woo, Kaichi Ohashi, Toshiki Suzuki, Akiko Kaneko, Atushi Hoshino, Ali Zada, Rose Baker, Michael Douek, Moriaki Kusakabe, Masaki Sekino

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The magnetic technique for sentinel node biopsy provides a radioisotope-free alternative for staging breast cancer. It requires refinement to reduce "residual iron content" at injection sites by maximising lymphatic uptake to prevent "void artefacts" on magnetic resonance imaging (MRI), which could adversely affect clinical use. The site and timing of injection of magnetic tracer was evaluated in a murine tumour model (right hind limb) in 24 wild type mice. Right-sided intratumoural and left sided subcutaneous injection of magnetic tracer and assessment of nodal iron uptake on MRI, surgical excision and histopathological grading at time frames up to 24 hours were performed. Rapid iron uptake on MRI, smaller "void artefacts"(P <0.001) and a significant increase in iron content with time were identified in the subcutaneous injection group (r = 0.937; P <0.001).Subcutaneous injection and increasing delay between tracer injection and surgery is beneficial for lymphatic iron uptake.

Original languageEnglish
Pages (from-to)1045-1052
Number of pages8
JournalNanomedicine-Nanotechnology Biology And Medicine
Volume12
Issue number4
Early online date28 Dec 2015
DOIs
Publication statusPublished - 1 May 2016

Keywords

  • Magnetic technique
  • Magnetic tracer
  • Murine tumour model
  • Sentinel lymph node biopsy
  • Superparamagnetic iron oxide (SPIO)

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