Magnetic sentinel lymph node biopsy in a murine tumour model

Muneer Ahmed; Taeseong Woo; Kaichi Ohashi; Toshiki Suzuki; Akiko Kaneko; Atushi Hoshino; Ali Zada; Rose Baker; Michael Douek; Moriaki Kusakabe; Masaki Sekino

doi:10.1016/j.nano.2015.12.366

Magnetic sentinel lymph node biopsy in a murine tumour model

Muneer Ahmed^*, Taeseong Woo, Kaichi Ohashi, Toshiki Suzuki, Akiko Kaneko, Atushi Hoshino, Ali Zada, Rose Baker, Michael Douek, Moriaki Kusakabe, Masaki Sekino

^*Corresponding author for this work

Comprehensive Cancer Centre

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The magnetic technique for sentinel node biopsy provides a radioisotope-free alternative for staging breast cancer. It requires refinement to reduce "residual iron content" at injection sites by maximising lymphatic uptake to prevent "void artefacts" on magnetic resonance imaging (MRI), which could adversely affect clinical use. The site and timing of injection of magnetic tracer was evaluated in a murine tumour model (right hind limb) in 24 wild type mice. Right-sided intratumoural and left sided subcutaneous injection of magnetic tracer and assessment of nodal iron uptake on MRI, surgical excision and histopathological grading at time frames up to 24 hours were performed. Rapid iron uptake on MRI, smaller "void artefacts"(P <0.001) and a significant increase in iron content with time were identified in the subcutaneous injection group (r = 0.937; P <0.001).Subcutaneous injection and increasing delay between tracer injection and surgery is beneficial for lymphatic iron uptake.

Original language	English
Pages (from-to)	1045-1052
Number of pages	8
Journal	Nanomedicine-Nanotechnology Biology And Medicine
Volume	12
Issue number	4
Early online date	28 Dec 2015
DOIs	https://doi.org/10.1016/j.nano.2015.12.366
Publication status	Published - 1 May 2016

Keywords

Magnetic technique
Magnetic tracer
Murine tumour model
Sentinel lymph node biopsy
Superparamagnetic iron oxide (SPIO)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.nano.2015.12.366

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title = "Magnetic sentinel lymph node biopsy in a murine tumour model",

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AU - Ahmed, Muneer

AU - Woo, Taeseong

AU - Ohashi, Kaichi

AU - Suzuki, Toshiki

AU - Kaneko, Akiko

AU - Hoshino, Atushi

AU - Zada, Ali

AU - Baker, Rose

AU - Douek, Michael

AU - Kusakabe, Moriaki

AU - Sekino, Masaki

PY - 2016/5/1

Y1 - 2016/5/1

N2 - The magnetic technique for sentinel node biopsy provides a radioisotope-free alternative for staging breast cancer. It requires refinement to reduce "residual iron content" at injection sites by maximising lymphatic uptake to prevent "void artefacts" on magnetic resonance imaging (MRI), which could adversely affect clinical use. The site and timing of injection of magnetic tracer was evaluated in a murine tumour model (right hind limb) in 24 wild type mice. Right-sided intratumoural and left sided subcutaneous injection of magnetic tracer and assessment of nodal iron uptake on MRI, surgical excision and histopathological grading at time frames up to 24 hours were performed. Rapid iron uptake on MRI, smaller "void artefacts"(P <0.001) and a significant increase in iron content with time were identified in the subcutaneous injection group (r = 0.937; P <0.001).Subcutaneous injection and increasing delay between tracer injection and surgery is beneficial for lymphatic iron uptake.

AB - The magnetic technique for sentinel node biopsy provides a radioisotope-free alternative for staging breast cancer. It requires refinement to reduce "residual iron content" at injection sites by maximising lymphatic uptake to prevent "void artefacts" on magnetic resonance imaging (MRI), which could adversely affect clinical use. The site and timing of injection of magnetic tracer was evaluated in a murine tumour model (right hind limb) in 24 wild type mice. Right-sided intratumoural and left sided subcutaneous injection of magnetic tracer and assessment of nodal iron uptake on MRI, surgical excision and histopathological grading at time frames up to 24 hours were performed. Rapid iron uptake on MRI, smaller "void artefacts"(P <0.001) and a significant increase in iron content with time were identified in the subcutaneous injection group (r = 0.937; P <0.001).Subcutaneous injection and increasing delay between tracer injection and surgery is beneficial for lymphatic iron uptake.

KW - Magnetic technique

KW - Magnetic tracer

KW - Murine tumour model

KW - Sentinel lymph node biopsy

KW - Superparamagnetic iron oxide (SPIO)

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JO - Nanomedicine-Nanotechnology Biology And Medicine

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ER -

Magnetic sentinel lymph node biopsy in a murine tumour model

Abstract

Keywords

UN SDGs

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