Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

Kay Richards; Fernando Calamante; Jacques-Donald Tournier; Nyoman D Kurniawan; Farnoosh Sadeghian; Alexander R Retchford; Gabriel Davis Jones; Christopher A Reid; David C Reutens; Roger Ordidge; Alan Connelly; Steven Petrou

doi:10.1016/j.neuroimage.2014.07.048

Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

Kay Richards, Fernando Calamante, Jacques-Donald Tournier, Nyoman D Kurniawan, Farnoosh Sadeghian, Alexander R Retchford, Gabriel Davis Jones, Christopher A Reid, David C Reutens, Roger Ordidge, Alan Connelly, Steven Petrou

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease.

Original language	English
Pages (from-to)	381-392
Number of pages	12
Journal	NeuroImage
Volume	102
Issue number	2
DOIs	https://doi.org/10.1016/j.neuroimage.2014.07.048
Publication status	Published - 15 Nov 2014

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Richards, K., Calamante, F., Tournier, J.-D., Kurniawan, N. D., Sadeghian, F., Retchford, A. R., Jones, G. D., Reid, C. A., Reutens, D. C., Ordidge, R., Connelly, A., & Petrou, S. (2014). Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging. NeuroImage, 102(2), 381-392. https://doi.org/10.1016/j.neuroimage.2014.07.048

@article{6879ce5e3cb3441c96fc942d3c90259b,

title = "Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging",

abstract = "In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease.",

author = "Kay Richards and Fernando Calamante and Jacques-Donald Tournier and Kurniawan, {Nyoman D} and Farnoosh Sadeghian and Retchford, {Alexander R} and Jones, {Gabriel Davis} and Reid, {Christopher A} and Reutens, {David C} and Roger Ordidge and Alan Connelly and Steven Petrou",

year = "2014",

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doi = "10.1016/j.neuroimage.2014.07.048",

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Richards, K, Calamante, F, Tournier, J-D, Kurniawan, ND, Sadeghian, F, Retchford, AR, Jones, GD, Reid, CA, Reutens, DC, Ordidge, R, Connelly, A & Petrou, S 2014, 'Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging', NeuroImage, vol. 102, no. 2, pp. 381-392. https://doi.org/10.1016/j.neuroimage.2014.07.048

TY - JOUR

T1 - Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

AU - Richards, Kay

AU - Calamante, Fernando

AU - Tournier, Jacques-Donald

AU - Kurniawan, Nyoman D

AU - Sadeghian, Farnoosh

AU - Retchford, Alexander R

AU - Jones, Gabriel Davis

AU - Reid, Christopher A

AU - Reutens, David C

AU - Ordidge, Roger

AU - Connelly, Alan

AU - Petrou, Steven

PY - 2014/11/15

Y1 - 2014/11/15

N2 - In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease.

AB - In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease.

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DO - 10.1016/j.neuroimage.2014.07.048

M3 - Article

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SN - 1053-8119

VL - 102

SP - 381

EP - 392

JO - NeuroImage

JF - NeuroImage

IS - 2

ER -

Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

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