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Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes

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Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes. / Harms, Matthew J.; Li, Qian; Lee, Sunjae; Zhang, Cheng; Kull, Bengt; Hallen, Stefan; Thorell, Anders; Alexandersson, Ida; Hagberg, Carolina E.; Peng, Xiao-Rong; Mardinoglu, Adil; Spalding, Kirsty L.; Boucher, Jeremie.

In: Cell Reports, Vol. 27, No. 1, 02.04.2019, p. 213-225.

Research output: Contribution to journalArticle

Harvard

Harms, MJ, Li, Q, Lee, S, Zhang, C, Kull, B, Hallen, S, Thorell, A, Alexandersson, I, Hagberg, CE, Peng, X-R, Mardinoglu, A, Spalding, KL & Boucher, J 2019, 'Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes', Cell Reports, vol. 27, no. 1, pp. 213-225. https://doi.org/10.1016/j.celrep.2019.03.026

APA

Harms, M. J., Li, Q., Lee, S., Zhang, C., Kull, B., Hallen, S., ... Boucher, J. (2019). Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes. Cell Reports, 27(1), 213-225. https://doi.org/10.1016/j.celrep.2019.03.026

Vancouver

Harms MJ, Li Q, Lee S, Zhang C, Kull B, Hallen S et al. Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes. Cell Reports. 2019 Apr 2;27(1):213-225. https://doi.org/10.1016/j.celrep.2019.03.026

Author

Harms, Matthew J. ; Li, Qian ; Lee, Sunjae ; Zhang, Cheng ; Kull, Bengt ; Hallen, Stefan ; Thorell, Anders ; Alexandersson, Ida ; Hagberg, Carolina E. ; Peng, Xiao-Rong ; Mardinoglu, Adil ; Spalding, Kirsty L. ; Boucher, Jeremie. / Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes. In: Cell Reports. 2019 ; Vol. 27, No. 1. pp. 213-225.

Bibtex Download

@article{f7b6b5b744cc42d68d1266017f822e3c,
title = "Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes",
abstract = "White adipose tissue (WAT) is a central factor in the development of type 2 diabetes, but there is a paucity of translational models to study mature adipocytes. We describe a method for the culture of mature white adipocytes under a permeable membrane. Compared to existing culture methods, MAAC (membrane mature adipocyte aggregate cultures) better maintain adipogenic gene expression, do not dedifferentiate, display reduced hypoxia, and remain functional after long-term culture. Subcutaneous and visceral adipocytes cultured as MAAC retain depot-specific gene expression, and adipocytes from both lean and obese patients can be cultured. Importantly, we show that rosiglitazone treatment or PGC1α overexpression in mature white adipocytes induces a brown fat transcriptional program, providing direct evidence that human adipocytes can transdifferentiate into brown-like adipocytes. Together, these data show that MAAC are a versatile tool for studying phenotypic changes of mature adipocytes and provide an improved translational model for drug development.",
keywords = "adipocyte, white adipose, WAT, brown adipose, BAT, transdifferentiation, UCP1, culture, MAAC",
author = "Harms, {Matthew J.} and Qian Li and Sunjae Lee and Cheng Zhang and Bengt Kull and Stefan Hallen and Anders Thorell and Ida Alexandersson and Hagberg, {Carolina E.} and Xiao-Rong Peng and Adil Mardinoglu and Spalding, {Kirsty L.} and Jeremie Boucher",
year = "2019",
month = "4",
day = "2",
doi = "10.1016/j.celrep.2019.03.026",
language = "English",
volume = "27",
pages = "213--225",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Elsevier BV",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes

AU - Harms, Matthew J.

AU - Li, Qian

AU - Lee, Sunjae

AU - Zhang, Cheng

AU - Kull, Bengt

AU - Hallen, Stefan

AU - Thorell, Anders

AU - Alexandersson, Ida

AU - Hagberg, Carolina E.

AU - Peng, Xiao-Rong

AU - Mardinoglu, Adil

AU - Spalding, Kirsty L.

AU - Boucher, Jeremie

PY - 2019/4/2

Y1 - 2019/4/2

N2 - White adipose tissue (WAT) is a central factor in the development of type 2 diabetes, but there is a paucity of translational models to study mature adipocytes. We describe a method for the culture of mature white adipocytes under a permeable membrane. Compared to existing culture methods, MAAC (membrane mature adipocyte aggregate cultures) better maintain adipogenic gene expression, do not dedifferentiate, display reduced hypoxia, and remain functional after long-term culture. Subcutaneous and visceral adipocytes cultured as MAAC retain depot-specific gene expression, and adipocytes from both lean and obese patients can be cultured. Importantly, we show that rosiglitazone treatment or PGC1α overexpression in mature white adipocytes induces a brown fat transcriptional program, providing direct evidence that human adipocytes can transdifferentiate into brown-like adipocytes. Together, these data show that MAAC are a versatile tool for studying phenotypic changes of mature adipocytes and provide an improved translational model for drug development.

AB - White adipose tissue (WAT) is a central factor in the development of type 2 diabetes, but there is a paucity of translational models to study mature adipocytes. We describe a method for the culture of mature white adipocytes under a permeable membrane. Compared to existing culture methods, MAAC (membrane mature adipocyte aggregate cultures) better maintain adipogenic gene expression, do not dedifferentiate, display reduced hypoxia, and remain functional after long-term culture. Subcutaneous and visceral adipocytes cultured as MAAC retain depot-specific gene expression, and adipocytes from both lean and obese patients can be cultured. Importantly, we show that rosiglitazone treatment or PGC1α overexpression in mature white adipocytes induces a brown fat transcriptional program, providing direct evidence that human adipocytes can transdifferentiate into brown-like adipocytes. Together, these data show that MAAC are a versatile tool for studying phenotypic changes of mature adipocytes and provide an improved translational model for drug development.

KW - adipocyte

KW - white adipose

KW - WAT

KW - brown adipose

KW - BAT

KW - transdifferentiation

KW - UCP1

KW - culture

KW - MAAC

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85063422988&origin=SingleRecordEmailAlert&dgcid=raven_sc_search_en_us_email&txGid=a03f9b01426e0cf28b40f11b241dce9e

U2 - 10.1016/j.celrep.2019.03.026

DO - 10.1016/j.celrep.2019.03.026

M3 - Article

VL - 27

SP - 213

EP - 225

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 1

ER -

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