Measuring diffusion exchange across the cell membrane with DEXSY (Diffusion Exchange Spectroscopy)

James O. Breen-Norris; Bernard Siow; Claire Walsh; Ben Hipwell; Ioana Hill; Thomas Roberts; Matt G. Hall; Mark F. Lythgoe; Andrada Ianus; Daniel C. Alexander; Simon Walker-Samuel

doi:10.1002/mrm.28207

Measuring diffusion exchange across the cell membrane with DEXSY (Diffusion Exchange Spectroscopy)

James O. Breen-Norris, Bernard Siow^*, Claire Walsh, Ben Hipwell, Ioana Hill, Thomas Roberts, Matt G. Hall, Mark F. Lythgoe, Andrada Ianus, Daniel C. Alexander, Simon Walker-Samuel

^*Corresponding author for this work

Biomedical Engineering Department

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Introduction: To combine numerical simulations, in vitro and in vivo experiments to evaluate the feasibility of measuring diffusion exchange across the cell membrane with diffusion exchange spectroscopy (DEXSY). Methods: DEXSY acquisitions were simulated over a range of permeabilities in nerve tissue and yeast substrates. In vitro measurements were performed in a yeast substrate and in vivo measurements in mouse tumor xenograft models, all at 9.4 T. Results: Diffusion exchange was observed in simulations over a physiologically relevant range of cell permeability values. In vitro and in vivo measures also provided evidence of diffusion exchange, which was quantified with the Diffusion Exchange Index (DEI). Conclusions: Our findings provide preliminary evidence that DEXSY can be used to make in vivo measurements of diffusion exchange and cell membrane permeability.

Original language	English
Pages (from-to)	1543-1551
Number of pages	9
Journal	Magnetic Resonance in Medicine
Volume	84
Issue number	3
DOIs	https://doi.org/10.1002/mrm.28207
Publication status	Published - 1 Sept 2020

Keywords

cell membrane permeability
DEXSY
Diffusion exchange
FEXSY

Access to Document

10.1002/mrm.28207

Cite this

@article{942f8540e98a4fd6b24cf9930d4c061f,

title = "Measuring diffusion exchange across the cell membrane with DEXSY (Diffusion Exchange Spectroscopy)",

abstract = "Introduction: To combine numerical simulations, in vitro and in vivo experiments to evaluate the feasibility of measuring diffusion exchange across the cell membrane with diffusion exchange spectroscopy (DEXSY). Methods: DEXSY acquisitions were simulated over a range of permeabilities in nerve tissue and yeast substrates. In vitro measurements were performed in a yeast substrate and in vivo measurements in mouse tumor xenograft models, all at 9.4 T. Results: Diffusion exchange was observed in simulations over a physiologically relevant range of cell permeability values. In vitro and in vivo measures also provided evidence of diffusion exchange, which was quantified with the Diffusion Exchange Index (DEI). Conclusions: Our findings provide preliminary evidence that DEXSY can be used to make in vivo measurements of diffusion exchange and cell membrane permeability.",

keywords = "cell membrane permeability, DEXSY, Diffusion exchange, FEXSY",

author = "Breen-Norris, {James O.} and Bernard Siow and Claire Walsh and Ben Hipwell and Ioana Hill and Thomas Roberts and Hall, {Matt G.} and Lythgoe, {Mark F.} and Andrada Ianus and Alexander, {Daniel C.} and Simon Walker-Samuel",

year = "2020",

month = sep,

day = "1",

doi = "10.1002/mrm.28207",

language = "English",

volume = "84",

pages = "1543--1551",

journal = "Magnetic Resonance in Medicine",

issn = "0740-3194",

publisher = "WILEY-BLACKWELL",

number = "3",

}

TY - JOUR

T1 - Measuring diffusion exchange across the cell membrane with DEXSY (Diffusion Exchange Spectroscopy)

AU - Breen-Norris, James O.

AU - Siow, Bernard

AU - Walsh, Claire

AU - Hipwell, Ben

AU - Hill, Ioana

AU - Roberts, Thomas

AU - Hall, Matt G.

AU - Lythgoe, Mark F.

AU - Ianus, Andrada

AU - Alexander, Daniel C.

AU - Walker-Samuel, Simon

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Introduction: To combine numerical simulations, in vitro and in vivo experiments to evaluate the feasibility of measuring diffusion exchange across the cell membrane with diffusion exchange spectroscopy (DEXSY). Methods: DEXSY acquisitions were simulated over a range of permeabilities in nerve tissue and yeast substrates. In vitro measurements were performed in a yeast substrate and in vivo measurements in mouse tumor xenograft models, all at 9.4 T. Results: Diffusion exchange was observed in simulations over a physiologically relevant range of cell permeability values. In vitro and in vivo measures also provided evidence of diffusion exchange, which was quantified with the Diffusion Exchange Index (DEI). Conclusions: Our findings provide preliminary evidence that DEXSY can be used to make in vivo measurements of diffusion exchange and cell membrane permeability.

AB - Introduction: To combine numerical simulations, in vitro and in vivo experiments to evaluate the feasibility of measuring diffusion exchange across the cell membrane with diffusion exchange spectroscopy (DEXSY). Methods: DEXSY acquisitions were simulated over a range of permeabilities in nerve tissue and yeast substrates. In vitro measurements were performed in a yeast substrate and in vivo measurements in mouse tumor xenograft models, all at 9.4 T. Results: Diffusion exchange was observed in simulations over a physiologically relevant range of cell permeability values. In vitro and in vivo measures also provided evidence of diffusion exchange, which was quantified with the Diffusion Exchange Index (DEI). Conclusions: Our findings provide preliminary evidence that DEXSY can be used to make in vivo measurements of diffusion exchange and cell membrane permeability.

KW - cell membrane permeability

KW - DEXSY

KW - Diffusion exchange

KW - FEXSY

UR - http://www.scopus.com/inward/record.url?scp=85079456086&partnerID=8YFLogxK

U2 - 10.1002/mrm.28207

DO - 10.1002/mrm.28207

M3 - Article

C2 - 32060975

AN - SCOPUS:85079456086

SN - 0740-3194

VL - 84

SP - 1543

EP - 1551

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

IS - 3

ER -

Measuring diffusion exchange across the cell membrane with DEXSY (Diffusion Exchange Spectroscopy)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this