Measuring Erosive Enamel Loss In Vitro After Single-Application Surface Treatments

Rupert Austin, Ryan Olley, Jose Rodriguez, Rebecca Moazzez, David Bartlett

Research output: Contribution to journalMeeting abstractpeer-review


Title: Measuring erosive enamel loss in vitro after single-application surface treatments
Objectives: To compare a novel profilometry technique for investigating the effect of surface treatments on the volume of enamel loss from multiple cycles of erosion-attrition in vitro.
Methods: A novel surface analysis technique was developed for non-destructive repeat measurements of enamel surfaces using a white-light confocal profilometer. The measurement technique was used to investigate the effect of two sodium fluoride varnishes and a resin-based adhesive on erosion-attrition in vitro. Polished human enamel specimens were pretreated with one of four treatments [G1=45200 ppm F varnish (Bifluoride 10®, Voco Gmbh, Cuxhaven, Germany); G2:22600 ppm F varnish (Duraphat® varnish, Colgate-Palmolive, Piscataway, USA); G3: self etch adhesive (Adper™ Prompt™ Self-Etch Adhesive (3M ESPE, St. Paul, MN, USA); G4: distilled water negative control]. All samples were subjected to 15 cycles of simulated erosion-attrition prior to measurement using white-light confocal profilometry. The conventional technique measured both loss of coating and loss of enamel together whereas the novel technique digitally removed the coating from the surface prior to measurement of enamel loss only.
Results: The novel technique quantified volume loss of enamel without the need to remove the surface coating prior to measurement, whereas the conventional measurement outcomes were complicated by the loss of product coating as well as enamel loss. The novel technique revealed that initially, both the fluoride varnishes and self-etch adhesive reduced enamel volume loss in comparison to control (P<0.01). However, after 15 cycles of erosion-attrition, whilst the fluoride varnishes did statistically significantly reduce enamel volume loss (P<0.01), the self-etch adhesive did not.
Conclusions: The non-destructive nature of the novel measurement technique allowed the surface coatings to be left in situ during measurement whilst multiple cycles of erosive wear and repeated measurements were made. Clinically relevant data were collected regarding the potential effects of fluoride containing surface treatments to modify erosive wear of enamel.
Original languageEnglish
JournalJournal of Dental Research
Issue numberSpec Iss B
Publication statusPublished - 2012


Dive into the research topics of 'Measuring Erosive Enamel Loss In Vitro After Single-Application Surface Treatments'. Together they form a unique fingerprint.

Cite this