Measuring PPFD on Algal Shaker

Research output: Contribution to journalArticle

Abstract

The illuminated orbital shaker features a glowing growing area. The illumination is not entirely homogeneous. It is brightest near the centers of the LEDs and becomes dimmer closer to the edges. The light brightness of the light in the glowing area is dependent on the LEDs, their spacing, the distance between the LEDs and the transparent platform, the material of the anti-slip mat, among other parameters. To ensure consistency and reproducibility between experiments, the brightness can be measured and the light output of the illuminator calibrated. Quantum detector is used to measure the photosynthetic photon flux density (PPFD). The measurement described in this protocol is conducted for all current settings on the LED controller and in 12 different locations across the glowing area. It is done both in air and inside an Erlenmeyer flask containing water, to best capture the actual conditions experienced by the microalgae cultures.
Original languageEnglish
Number of pages7
Journalprotocols.io
DOIs
Publication statusPublished - 20 Mar 2020

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