Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

Balaraman Kalyanaraman; Victor Darley-Usmar; Kelvin J. A. Davies; Phyllis A. Dennery; Henry Jay Forman; Matthew B. Grisham; Giovanni E. Mann; Kevin Moore; L. Jackson Roberts; Harry Ischiropoulos

doi:10.1016/j.freeradbiomed.2011.09.030

Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

Balaraman Kalyanaraman, Victor Darley-Usmar, Kelvin J. A. Davies, Phyllis A. Dennery, Henry Jay Forman, Matthew B. Grisham, Giovanni E. Mann, Kevin Moore, L. Jackson Roberts, Harry Ischiropoulos

Vascular Biology & Inflammation

Research output: Contribution to journal › Editorial › peer-review

1460 Citations (Scopus)

Abstract

The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results. (C) 2011 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	1 - 6
Number of pages	6
Journal	Free Radical Biology and Medicine
Volume	52
Issue number	1
DOIs	https://doi.org/10.1016/j.freeradbiomed.2011.09.030
Publication status	Published - 1 Jan 2012

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10.1016/j.freeradbiomed.2011.09.030

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title = "Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations",

abstract = "The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results. (C) 2011 Elsevier Inc. All rights reserved.",

author = "Balaraman Kalyanaraman and Victor Darley-Usmar and Davies, {Kelvin J. A.} and Dennery, {Phyllis A.} and Forman, {Henry Jay} and Grisham, {Matthew B.} and Mann, {Giovanni E.} and Kevin Moore and Roberts, {L. Jackson} and Harry Ischiropoulos",

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AU - Kalyanaraman, Balaraman

AU - Darley-Usmar, Victor

AU - Davies, Kelvin J. A.

AU - Dennery, Phyllis A.

AU - Forman, Henry Jay

AU - Grisham, Matthew B.

AU - Mann, Giovanni E.

AU - Moore, Kevin

AU - Roberts, L. Jackson

AU - Ischiropoulos, Harry

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N2 - The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results. (C) 2011 Elsevier Inc. All rights reserved.

AB - The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results. (C) 2011 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.freeradbiomed.2011.09.030

DO - 10.1016/j.freeradbiomed.2011.09.030

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JF - Free Radical Biology and Medicine

IS - 1

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Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

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