Method for protein subfractionation of cardiovascular tissues before DIGE analysis

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Difference gel electrophoresis (DIGE) (Electrophoresis 18, 2071-2077, 1997, 1) is widely used in cardiovascular research. However, the dynamic range limitations stemming from contaminating plasma proteins and highly abundant extracellular matrix components can make cardiovascular tissues difficult to analyze. Here we describe a novel methodology for biochemical subfractionation of cardiovascular tissues before DIGE analysis.
Original languageEnglish
Title of host publicationDifference Gel Electrophoresis (DIGE)
Subtitle of host publicationMethods and Protocols
EditorsRainer Cramer, Reiner Westermeier
PublisherHumana Press
Pages287-297
Number of pages11
Volume854
ISBN (Electronic)978-1-61779-573-2
ISBN (Print)978-1-61779-572-5
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume854
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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