Michel's medium: a potential alternative to cryoprotection for tissue transport in the investigation of genetic skin disease

TY - JOUR

T1 - Michel's medium: a potential alternative to cryoprotection for tissue transport in the investigation of genetic skin disease

AU - Woollons, A

AU - Holmes, G J

AU - Gratian, M J

AU - Bhogal, B S

AU - Black, M M

PY - 1999

Y1 - 1999

N2 - Michel's medium is now well established as a transport medium to maintain tissue-fixed immunoreactants prior to direct immunofluorescence and immunoelectron microscopy. This is a practical alternative to cryofixation prior to transportation when sending skin biopsies for the investigation of cutaneous immunobullous disease. In this study we have demonstrated preservation of the cutaneous basement membrane zone proteins in skin biopsies stored in Michel's medium for periods of up to 28 days - proving that Michel's medium can be used as a transport medium when sending skin biopsies for immunohistochemical studies to determine the structural and molecular deficiencies in genodermatoses such as inherited forms of epidermolysis bullosa.

AB - Michel's medium is now well established as a transport medium to maintain tissue-fixed immunoreactants prior to direct immunofluorescence and immunoelectron microscopy. This is a practical alternative to cryofixation prior to transportation when sending skin biopsies for the investigation of cutaneous immunobullous disease. In this study we have demonstrated preservation of the cutaneous basement membrane zone proteins in skin biopsies stored in Michel's medium for periods of up to 28 days - proving that Michel's medium can be used as a transport medium when sending skin biopsies for immunohistochemical studies to determine the structural and molecular deficiencies in genodermatoses such as inherited forms of epidermolysis bullosa.

UR - http://www.scopus.com/inward/record.url?scp=0033428438&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2230.1999.00540.x

DO - 10.1046/j.1365-2230.1999.00540.x

M3 - Article

SN - 1365-2230

VL - 24

SP - 487

EP - 489

JO - Clinical and Experimental Dermatology

JF - Clinical and Experimental Dermatology

IS - 6

ER -