TY - JOUR
T1 - Microbial community compositions and sulfate-reducing bacterial profiles in malodorous urban canal sediments
AU - Jantharadej, Krittayapong
AU - Limpiyakorn, Tawan
AU - Kongprajug, Akechai
AU - Mongkolsuk, Skorn
AU - Sirikanchana, Kwanrawee
AU - Suwannasilp, Benjaporn Boonchayaanant
N1 - Funding Information:
This study was funded by Chulalongkorn University through the Special Task Force for Activating Research (STAR) program and the Chulabhorn Research Institute, Bangkok, Thailand.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
PY - 2021/7
Y1 - 2021/7
N2 - Anthropogenically impacted urban canals represent distinct freshwater ecosystems that could shape microbial communities in underlying sediments; however, knowledge of the relationships between environmental factors and microbial community compositions and their functions in such an environment is limited. This study characterized the microbial community compositions of malodorous canal sediments at six locations along the Saen Saep Canal in Thailand. 16S rRNA gene amplicon sequencing (MiSeq, Illumina) revealed dominant genera classified as fermentative bacteria, methanogens, and sulfate-reducing bacteria (SRB), all of which emphasized anaerobic environments. SRB, as the primary producers of malodorous hydrogen sulfide, accounted for 8.2–30.4% of the total sequences. dsrB gene clone libraries further identified the SRB species. A constrained correspondence analysis demonstrated a spatial pattern of SRB that correlated with physicochemical parameters in which nitrate and sulfate in sediments were the most influencing factors. Overall, a better understanding of the SRB and other related microorganisms in canal sediments can assist in the future implementation of appropriate olfactory abatement and management methodologies in urban canals.
AB - Anthropogenically impacted urban canals represent distinct freshwater ecosystems that could shape microbial communities in underlying sediments; however, knowledge of the relationships between environmental factors and microbial community compositions and their functions in such an environment is limited. This study characterized the microbial community compositions of malodorous canal sediments at six locations along the Saen Saep Canal in Thailand. 16S rRNA gene amplicon sequencing (MiSeq, Illumina) revealed dominant genera classified as fermentative bacteria, methanogens, and sulfate-reducing bacteria (SRB), all of which emphasized anaerobic environments. SRB, as the primary producers of malodorous hydrogen sulfide, accounted for 8.2–30.4% of the total sequences. dsrB gene clone libraries further identified the SRB species. A constrained correspondence analysis demonstrated a spatial pattern of SRB that correlated with physicochemical parameters in which nitrate and sulfate in sediments were the most influencing factors. Overall, a better understanding of the SRB and other related microorganisms in canal sediments can assist in the future implementation of appropriate olfactory abatement and management methodologies in urban canals.
KW - 16S rRNA gene amplicon sequencing
KW - Anaerobic condition
KW - Constrained correspondence analysis
KW - dsrB gene clone library
KW - Water pollution
UR - http://www.scopus.com/inward/record.url?scp=85100334914&partnerID=8YFLogxK
U2 - 10.1007/s00203-020-02157-7
DO - 10.1007/s00203-020-02157-7
M3 - Article
C2 - 33528590
AN - SCOPUS:85100334914
SN - 0302-8933
VL - 203
SP - 1981
EP - 1993
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 5
ER -
