TY - JOUR
T1 - Microbiological and molecular profile of furcation defects in a population with untreated periodontitis
AU - Santamaria, Pasquale
AU - Jin, Yi
AU - Ghuman, Mandeep
AU - Shoaie, Saeed
AU - Spratt, David
AU - Troiano, Giuseppe
AU - Nibali, Luigi
N1 - Publisher Copyright:
© 2024 The Author(s). Journal of Clinical Periodontology published by John Wiley & Sons Ltd.
PY - 2024/11
Y1 - 2024/11
N2 - Aim: To describe the microbiological composition of subgingival dental plaque and molecular profile of gingival crevicular fluid (GCF) of periodontal furcation-involved defects. Materials and Methods: Fifty-seven participants with periodontitis contributed with a degree II–III furcation involvement (FI), a non-furcation (NF) periodontal defect and a periodontally healthy site (HS). Subgingival plaque was analysed by sequencing the V3–V4 region of the 16S rRNA gene, and a multiplex bead immunoassay was carried out to estimate the GCF levels of 18 GCF biomarkers. Aiming to explore inherent patterns and the intrinsic structure of data, an AI-clustering method was also applied. Results: In total, 171 subgingival plaque and 84 GCF samples were analysed. Four microbiome clusters were identified and associated with FI, NF and HS. A reduced aerobic microbiota (p =.01) was detected in FI compared with NF; IL-6, MMP-3, MMP-8, BMP-2, SOST, EGF and TIMP-1 levels were increased in the GCF of FI compared with NF. Conclusions: This is the first study to profile periodontal furcation defects from a microbiological and inflammatory standpoint using conventional and AI-based analyses. A reduced aerobic microbial biofilm and an increase of several inflammatory, connective tissue degradation and repair markers were detected compared with other periodontal defects.
AB - Aim: To describe the microbiological composition of subgingival dental plaque and molecular profile of gingival crevicular fluid (GCF) of periodontal furcation-involved defects. Materials and Methods: Fifty-seven participants with periodontitis contributed with a degree II–III furcation involvement (FI), a non-furcation (NF) periodontal defect and a periodontally healthy site (HS). Subgingival plaque was analysed by sequencing the V3–V4 region of the 16S rRNA gene, and a multiplex bead immunoassay was carried out to estimate the GCF levels of 18 GCF biomarkers. Aiming to explore inherent patterns and the intrinsic structure of data, an AI-clustering method was also applied. Results: In total, 171 subgingival plaque and 84 GCF samples were analysed. Four microbiome clusters were identified and associated with FI, NF and HS. A reduced aerobic microbiota (p =.01) was detected in FI compared with NF; IL-6, MMP-3, MMP-8, BMP-2, SOST, EGF and TIMP-1 levels were increased in the GCF of FI compared with NF. Conclusions: This is the first study to profile periodontal furcation defects from a microbiological and inflammatory standpoint using conventional and AI-based analyses. A reduced aerobic microbial biofilm and an increase of several inflammatory, connective tissue degradation and repair markers were detected compared with other periodontal defects.
KW - dental plaque
KW - gingival crevicular fluid
KW - microbiology
KW - periodontal bone loss
KW - periodontitis
UR - http://www.scopus.com/inward/record.url?scp=85200476267&partnerID=8YFLogxK
U2 - 10.1111/jcpe.14034
DO - 10.1111/jcpe.14034
M3 - Article
AN - SCOPUS:85200476267
SN - 0303-6979
VL - 51
SP - 1421
EP - 1432
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 11
ER -