Microinjection techniques for studying centrosome function in Drosophila melanogaster syncytial embryos

Paul T. Conduit*, Daniel Hayward, James G. Wakefield

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Microinjection is a powerful technique that can be used to study protein function. Early Drosophila embryos are particularly amenable to microinjection due to their large size and their single cell status. Here, we report methods to microinject these embryos with various reagents to study the function of proteins at centrosomes and centrosome function more generally. Although precise details vary between laboratories, many aspects of the process are conserved. We describe the process from setting up a fly cage to imaging the injected embryos on a spinning disk confocal microscope and use specific examples to highlight the potency of this technique.

Original languageEnglish
Title of host publicationMethods in Cell Biology
Subtitle of host publicationCentrosome
EditorsKaren Oegema, Renata Basto
PublisherACADEMIC PRESS INC
Pages229-249
Number of pages21
ISBN (Print)9780128024492
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Cell Biology
Volume129
ISSN (Print)0091-679X

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