MicroRNA Biomarkers and Platelet Reactivity: The Clot Thickens

Nicholas Sunderland; Philipp Skroblin; Temo Barwari; Rachael P. Huntley; Ruifang Lu; Abhishek Joshi; Ruth C. Lovering; Manuel Mayr

doi:10.1161/CIRCRESAHA.116.309303

MicroRNA Biomarkers and Platelet Reactivity: The Clot Thickens

Nicholas Sunderland, Philipp Skroblin, Temo Barwari, Rachael P. Huntley, Ruifang Lu, Abhishek Joshi, Ruth C. Lovering, Manuel Mayr^*

^*Corresponding author for this work

UCL University College London

Research output: Contribution to journal › Review article › peer-review

162 Citations (Scopus)

422 Downloads (Pure)

Abstract

Over the last few years, several groups have evaluated the potential of microRNAs (miRNAs) as biomarkers for cardiometabolic disease. In this review, we discuss the emerging literature on the role of miRNAs and other small noncoding RNAs in platelets and in the circulation, and the potential use of miRNAs as biomarkers for platelet activation. Platelets are a major source of miRNAs, YRNAs, and circular RNAs. By harnessing multiomics approaches, we may gain valuable insights into their potential function. Because not all miRNAs are detectable in the circulation, we also created a gene ontology annotation for circulating miRNAs using the gene ontology term extracellular space as part of blood plasma. Finally, we share key insights for measuring circulating miRNAs. We propose ways to standardize miRNA measurements, in particular by using platelet-poor plasma to avoid confounding caused by residual platelets in plasma or by adding RNase inhibitors to serum to reduce degradation. This should enhance comparability of miRNA measurements across different cohorts. We provide recommendations for future miRNA biomarker studies, emphasizing the need for accurate interpretation within a biological and methodological context.

Original language	English
Pages (from-to)	418-435
Number of pages	18
Journal	Circulation Research
Volume	120
Issue number	2
DOIs	https://doi.org/10.1161/CIRCRESAHA.116.309303
Publication status	Published - 20 Jan 2017

Keywords

acute coronary syndrome
biomarker
noncoding RNA
platelet
platelet inhibitor

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10.1161/CIRCRESAHA.116.309303

MicroRNA Biomarkers and_SUNDERLAND_Published20January2017_GREEN AAMAccepted author manuscript, 1.66 MB

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title = "MicroRNA Biomarkers and Platelet Reactivity: The Clot Thickens",

abstract = "Over the last few years, several groups have evaluated the potential of microRNAs (miRNAs) as biomarkers for cardiometabolic disease. In this review, we discuss the emerging literature on the role of miRNAs and other small noncoding RNAs in platelets and in the circulation, and the potential use of miRNAs as biomarkers for platelet activation. Platelets are a major source of miRNAs, YRNAs, and circular RNAs. By harnessing multiomics approaches, we may gain valuable insights into their potential function. Because not all miRNAs are detectable in the circulation, we also created a gene ontology annotation for circulating miRNAs using the gene ontology term extracellular space as part of blood plasma. Finally, we share key insights for measuring circulating miRNAs. We propose ways to standardize miRNA measurements, in particular by using platelet-poor plasma to avoid confounding caused by residual platelets in plasma or by adding RNase inhibitors to serum to reduce degradation. This should enhance comparability of miRNA measurements across different cohorts. We provide recommendations for future miRNA biomarker studies, emphasizing the need for accurate interpretation within a biological and methodological context.",

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doi = "10.1161/CIRCRESAHA.116.309303",

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AU - Sunderland, Nicholas

AU - Skroblin, Philipp

AU - Barwari, Temo

AU - Huntley, Rachael P.

AU - Lu, Ruifang

AU - Joshi, Abhishek

AU - Lovering, Ruth C.

AU - Mayr, Manuel

PY - 2017/1/20

Y1 - 2017/1/20

N2 - Over the last few years, several groups have evaluated the potential of microRNAs (miRNAs) as biomarkers for cardiometabolic disease. In this review, we discuss the emerging literature on the role of miRNAs and other small noncoding RNAs in platelets and in the circulation, and the potential use of miRNAs as biomarkers for platelet activation. Platelets are a major source of miRNAs, YRNAs, and circular RNAs. By harnessing multiomics approaches, we may gain valuable insights into their potential function. Because not all miRNAs are detectable in the circulation, we also created a gene ontology annotation for circulating miRNAs using the gene ontology term extracellular space as part of blood plasma. Finally, we share key insights for measuring circulating miRNAs. We propose ways to standardize miRNA measurements, in particular by using platelet-poor plasma to avoid confounding caused by residual platelets in plasma or by adding RNase inhibitors to serum to reduce degradation. This should enhance comparability of miRNA measurements across different cohorts. We provide recommendations for future miRNA biomarker studies, emphasizing the need for accurate interpretation within a biological and methodological context.

AB - Over the last few years, several groups have evaluated the potential of microRNAs (miRNAs) as biomarkers for cardiometabolic disease. In this review, we discuss the emerging literature on the role of miRNAs and other small noncoding RNAs in platelets and in the circulation, and the potential use of miRNAs as biomarkers for platelet activation. Platelets are a major source of miRNAs, YRNAs, and circular RNAs. By harnessing multiomics approaches, we may gain valuable insights into their potential function. Because not all miRNAs are detectable in the circulation, we also created a gene ontology annotation for circulating miRNAs using the gene ontology term extracellular space as part of blood plasma. Finally, we share key insights for measuring circulating miRNAs. We propose ways to standardize miRNA measurements, in particular by using platelet-poor plasma to avoid confounding caused by residual platelets in plasma or by adding RNase inhibitors to serum to reduce degradation. This should enhance comparability of miRNA measurements across different cohorts. We provide recommendations for future miRNA biomarker studies, emphasizing the need for accurate interpretation within a biological and methodological context.

KW - acute coronary syndrome

KW - biomarker

KW - noncoding RNA

KW - platelet

KW - platelet inhibitor

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DO - 10.1161/CIRCRESAHA.116.309303

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AN - SCOPUS:85009854458

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VL - 120

SP - 418

EP - 435

JO - Circulation Research

JF - Circulation Research

IS - 2

ER -

MicroRNA Biomarkers and Platelet Reactivity: The Clot Thickens

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Keywords

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