MicroRNAs control eyelid development through regulating Wnt signaling

Takahiro Nagai, Supaluk Trakanant, Maiko Kawasaki, Katsushige Kawasaki, Yurie Yamada, Momoko Watanabe, James Blackburn, Yoko Otsuka-Tanaka, Mitsue Hishinuma, Atsushi Kitatmura, Fumiya Meguro, Akane Yamada, Yasumitsu Kodama, Takeyasu Maeda, Qiliang Zhou, Yasuo Saijo, Akihiro Yasue, Paul T. Sharpe, Robert Hindges, Ritsuo TakagiAtsushi Ohazama*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
110 Downloads (Pure)


Background: The timing, location, and level of gene expression are crucial for normal organ development, because morphogenesis requires strict genetic control. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating gene expression level. Although miRNAs are known to be involved in many biological events, the role of miRNAs in organogenesis is not fully understood. Mammalian eyelids fuse and separate during development and growth. In mice, failure of this process results in the eye-open at birth (EOB) phenotype. Results: It has been shown that conditional deletion of mesenchymal Dicer (an essential protein for miRNA processing; Dicerfl/fl;Wnt1Cre) leads to the EOB phenotype with full penetrance. Here, we identified that the up-regulation of Wnt signaling resulted in the EOB phenotype in Dicer mutants. Down-regulation of Fgf signaling observed in Dicer mutants was caused by an inverse relationship between Fgf and Wnt signaling. Shh and Bmp signaling were down-regulated as the secondary effects in Dicerfl/fl;Wnt1Cre mice. Wnt, Shh, and Fgf signaling were also found to mediate the epithelial–mesenchymal interactions in eyelid development.Conclusions: miRNAs control eyelid development through Wnt. Developmental Dynamics 2019.

Original languageEnglish
Pages (from-to)201-210
Number of pages10
JournalDevelopmental Dynamics
Issue number3
Early online date4 Feb 2019
Publication statusPublished - 1 Mar 2019


  • Bmp
  • dicer
  • eyelid development
  • Fgf
  • mesenchyme
  • microRNA
  • Shh
  • Wnt


Dive into the research topics of 'MicroRNAs control eyelid development through regulating Wnt signaling'. Together they form a unique fingerprint.

Cite this